Naslov
Vascular endothelial growth factor in relation to nuclear factor – kappa B in renal cell carcinoma

Autori
Đorđević, Gordana ; Matušan-Ilijaš, Koviljka ; Sinožić, Emina ; Licul, Vanja ; Grahovac, Blaženka ; Jonjić, Nives

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 21st European Congress of Pathology ; u: Virchows Archive 451 (2007) (5) ; PP4: Gynecopathology ; PP4-88 / - , 2007, 520-520

Skup
European Congress of Pathology (21 ; 2007)

Mjesto i datum
Istanbul, Turska, 08.09.2007. - 13.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
carcinoma; renal cell; nuclear Ffactor kappa B; immunohistochemistry; polymerase chain reaction; VEGF

Sažetak
Renal cell carcinoma (RCC) is a malignancy with variable clinical course, partly attributable to specific genetic alterations of the different RCC types. Angiogenesis is important for tumor progression and metastatic spread. VEGF is a major factor which regulates angiogenesis while the nuclear factorkappaB(NF-kB), a family of transcription factors ; regulate a wide variety of cellular processes including cell growth, differentiation and apoptosis. The aim of this study was to evaluate the expression level of VEGF and to compare their values with the subcellular localization of NF-kB, proliferative rate of tumor cells and clinicopathological characteristics of RCC. Immunohistochemical evaluation included VEGF expression, the subcellular localization of p65 member of NF-kB and Ki67 for proliferative rate of tumor cells in series of 16 RCC. Total RNA was extracted from the same tumor tissue samples, previously snap-frozen. Expression of VEGF was analyzed using quantitative Real-time PCR. The difference in VEGF mRNA levels between conventional (11 clear cell RCC) and other histological types of RCC was not significant. Preliminary results indicate a more pronounce heterogeneity in VEGF expression within the group of clear cell RCC. VEGF mRNA levels show some positive association with VEGF protein expression evaluated as the percentage of positive cells and intensity of staining within the cells, while there was no association with NF-kB and Ki67. Larger tumors were characterized with higher value of VEGF expression, NF-kB and Ki67 and, moreover, the proliferation rate of tumor cells was higher in tumors with higher VEGF expression. Preliminary results indicate the heterogeneity in VEGF expression in conventional type of RCC. The association with tumor progression (tumor size and proliferation rate) indicates that VEGF is an important angiogenic factor in RCC. Although no association between NF-kB activity, VEGF expression and Ki67 was found on a small number of analyzed samples, our preliminary data suggest that the NF-kB may be important factor in renal carcinogensis by controlling cells proliferation. These findings need further validation of usefulness of NF-kB as a prognostic factor in RCC.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

Napomena
DOI: 10.1007/s00428-007-0493-5

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