Naslov
Correlation of Adherence and Hemagglutination Abilities of Staphylococcus saprophyticus Strains

Autori
Vraneš, Jasmina ; Leskovar, Vladimira ; Bedenić, Branka ; Ljubin-Sternak, Sunčanica ; Mlinarić-Džepina, Ana ; Zvirotić, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) Abstracts, Clinical Microbiology and Infection Vol. 14, Suppl. 7 / - Oxford : Wiley-Blackwell, 2008

Skup
18th ECCMID

Mjesto i datum
Barcelona, Španjolska, 19.04.2008. - 22.04.2008

Vrsta sudjelovanja
Ostalo

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Staphylococcus saprophyticus; adherence ability; urinary tract infection; pathogenesis

Sažetak
Objectives: Bacterial adherence to the urinary mucosa is commonly regarded as the crucial initial step in the pathogenesis of urinary tract infections. The aim of this study was to compare the adherence ability of S. saprophyticus strains to the Buffalo green monkey kidney (BGMK) cell line with hemagglutination (HA) ability of those strains, isolated from the middle clean-catch urine samples of 60 young women. Methods: The strains were divided in two groups: thirty strains of studied strains were isolated in great number (>=10^4 CFU/ml) and pure culture from patients with urinary tract infection, and another thirty strains were isolated in small number (<=10^3 CFU/ml) in mixed culture. Examination of the HA activities with sheep erythrocytes was performed in microtitar plates, and for adhesion assay the BGMK cell line was used. Results: The results of the study showed that the strains isolated in great number and pure culture from the urine of women with urinary tract infection mostly express hemagglutinin (73% of the strains), have statistically significant (p<0.01) greater HA capability and adhere good or excellent to BGMK cell line in comparison with the strains isolated in small number and mixed culture which mostly did not express the hemagglutinin (only 36% of the strains), have statistically significant (p<0.01) smaller HA capability and did not adhered or adhered poorly to used cell line. In both studied groups of strains were noticed statistically significant correlation (rs=0.556, p=0.001 and rs=0.543, p=0.002) between the adherence and HA ability and greater adherence capability in strains with higher HA titers. Conclusions: This in vitro study showed statistical significant difference in virulence and pathogenicity between the two studied groups of strains. As the HA ability correlated with the adherence ability of strains, the simple and easy to perform HA test could be used in clinical laboratory for estimation of adherence ability and virulence potential of S. saprophyticus strains.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti

POVEZANOST RADA