Naslov
Lithium-pilocarpine status epilepticus in rat: structure-related changes in the brain antioxidant enzyme activities and lipid peroxidation

Autori
Peternel, Sandra ; Blagaić, Ana ; Pilipović, Kristina ; Hrelja, Ana ; Mršić-Pelčić, Jasenka ; Vitezić, Dinko ; Župan, Gordana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Drugi hrvatski kongres neuroznanosti : knjiga sažetaka = Abstracts of the Second Croatian Congress of Neuroscience ; u: Neurologia Croatica 56 (2007)(S2) / Ivkić, G ; Judaš, M ; Klarica, M ; Kostović, I ; Šimić, G, Petanjek, Z - Zagreb, 2007, 59-59

Skup
Hrvatski kongres neuroznanosti (2 ; 2007)

Mjesto i datum
Zagreb, Hrvatska, 18.05.2007. - 19.05.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
superoxide dismutase; glutathione peroxidase; lipid peroxidation; status epilepticus; rat

Sažetak
The lithium plus pilocarpine-induced status epilepticus (SE) triggers a cascade of events in the rat brain which leads to later development of spontaneous recurrent seizures resembling human temporal lobe epilepsy. The role of oxidative stress in seizure-induced neurodegeneration in this epilepsy model is not well characterized. Therefore, the aim of our study was to evaluate regional changes in the brain antioxidant enzyme activities as well as the level of lipid oxidative damage 24 hrs after induction of SE in rats. Experiments were performed on 80 to 90 days old male Wistar rats. SE was induced by administration of pilocarpine hydrochloride (30 mg/kg, i.p.) 22 hrs after LiCl (127 mg/kg, i.p) and 30 min after methylscopolamine nitrate (1 mg/kg, s.c.). SE was interrupted 2 hrs after its onset by an injection of diazepam (10 mg/kg, i.p.). Control animals received LiCl, saline and diazepam. Rats were sacrificed 24 hrs after the SE onset. Anterior olfactory nuclei, piriform/entorhinal cortex, temporal neocortex, thalamus and hippocampus were dissected and prepared for biochemical analyses. Regional brain antioxidant defense system was evaluated by spectrophotometric measurements of superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities. Levels of the brain lipid peroxidation were determined by thiobarbituric acid reactive substances (TBARS) assay. Lithium plus pilocarpine-induced SE resulted in statistically significant increases of both SOD (20.3%) and GPX (15.7%) activities in thalamus and of GPX activity (29.7%) in piriform/entorhinal cortex. In other brain structures examined, with the exception of hippocampus, a tendency of increase in the activities of both enzymes was observed. The TBARS levels significantly increased in anterior olfactory nuclei (11.8%), piriform/entorhinal cortex (58.3%), temporal neocortex (28.8%) and thalamus (30.1%), but remained unchanged in hippocampus. Lithium-pilocarpine SE results in structure-related activation of the enzymatic antioxidant system and causes extensive oxidative damage of lipids in the rat brain 24 hrs after its onset. Piriform/entorhinal cortex and thalamus are the most vulnerable brain structures in our experimental conditions.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA