Naslov
Differential sensitivity to 4-hydroxynonenal for normal and malignant mesenchymal cells

Autori
Borović, Suzana ; Čipak, Ana ; Meinitzer, Andreas ; Kejla, Zvonko ; Perović, Darko ; Waeg, Georg ; Žarković, Neven

Izvornik
Redox report (1351-0002) 12 (2007), 1/2; 50-54

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
4-hydroxynonenal; mesenchymal cells; human osteosarcoma; human osteoblasts; apoptosis; hne-his elisa; glutathione; persistent oxidative stress; human osteosarcoma cells; lipid-peroxidation; glutathione; conjugation; cancer; localization; products; adducts; tissues

Sažetak
Normal and malignant cells of various origin differ in their sensitivity to oxidative stress. Therefore, we used normal and malignant mesenchymal cells-human osteosarcoma cells (HOS and 143B), human fibroblasts (WI38) and two primary cultures of normal human osteoblasts to test sensitivity to reactive aldehyde 4- hydroxynonenal (HNE), known as a second messenger of free radicals and a signaling molecule. Upon HNE-treatment, decrease in cell viability (by Trypan-blue), apoptosis induction (by TiterTACS (TM) TUNEL assay), HNE-protein binding (by HNE-His ELISA) were higher in malignant than in normal cells, while glutathione content was higher in normal cells. These results indicate that HNE affects the growth of malignant mesenchymal cells more than normal and that this effect was mainly related to lower glutathione concentration and higher binding of HNE to the cellular proteins. We thus assume that HNE and GSH homeostasis play an important role in the growth regulation of normal and malignant mesenchymal cells.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA