Naslov
Possible Modulating Role of CD26 in Immune Response During Colitis Development in Mice

Autori
Detel, Dijana ; Kučić, Natalia ; Batičić, Lara ; Varljen, Jadranka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clinical Chemistry and Laboratory Medicine / Gerard Siest - Berlin : New York : Walter de Gruyter, 2008, A24-A24

Skup
3rd International Conference on Dipeptidyl Peptidase and Related Proteins

Mjesto i datum
Antwerpen, Belgija, 23.04.2008. - 25.04.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
CD26/DPP IV; immune response; colitis development CD 26-/- mice

Sažetak
Introduction: The CD26 molecule (dipeptidyl peptidase IV, DPP IV, EC 3.4.14.5.) is a widely expressed multifunctional transmembrane glycoprotein involved in hematopoesis and other biological function through T-cell activation and/or its proteolytic activity. Immunological abnormalities including the overproduction of proinflammatory cytokines and other immunological factors as well as potential role of CD26 in pathogenesis of chronic inflammatory diseases were suggested. In order to investigate etiopathogenesis of colitis the experimental murine model was established. Aim: The aim of this study was to investigate the potential role of CD26 in experimental murine model of colitis induced in C57BL/6 and CD26 deficient mice by characterization the immune phenotypic profile of blood, hepatic and splenic mononuclear lymphatic cells (MNLC). Material and methods: Male, homozygous CD26 deficient mice generated on a C57BL/6 genetic background and wild-type C57BL/6 mice were used in the study. Colitis was induced by administration of 3% (w/v) dextran sulfate sodium dissolved in drinking water with free access during 7 days. The changes in the percentage of CD3+, CD4+, CD8+, NK+, CD25+, CD122+, CD45+ and CD26+ MNLC in blood, liver and spleen were analyzed by flow-cytometry (FACSCalibur, Becton Dickinson). Data were evaluated using the Sigma Plot Scientific Graphing System, Version 6.10. The statistical significance was tested using Mann-Whitney U-test. The differences were considered significant for p<0.05. Results: The preliminary results of our study show that the expression of CD26 in acute phase (7th day) of the disease is statistically significantly decreased in comparison with healthy mice. The percentage of blood and spleen MNLC (CD3+, CD4+ and CD8+) in acute phase of the disease in CD26 deficient and C57BL/6 mice is statistically significantly decreased in comparison with healthy mice. Furthermore, the percentage of MNLC (CD3+, CD4+ and CD8+) in liver was statistically significantly increased in both mice groups in comparison to healthy mice. In CD26 deficient mice in acute phase of the disease the percentages of MNLC (CD3+, CD4+ and CD8+) are increased in comparison with C57BL/6 mice. During the recovery phase of the disease the increase in the percentage of CD122+ cells show the activation of Th1 response. Conclusion: Our data indicate the possible modulating role of CD26 molecule in the regulation of local and systemic immune response in acute phase of colitis, which makes it important in pathogenesis.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA