Naslov
Downregulation of TFFs encoding gastrointestinal healing peptides by Cytokines and Nuclear Factors

Autori
Baus Lončar, Mirela ; Kayademir, T ; Leistenschneider, P ; Machado, J.C ; Seitz, G ; Blin, N.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annual meeting of German pathology society, Book of abstracts / - , 2003

Skup
Annual meeting of German pathology society

Mjesto i datum
Bamberg, Njemačka, 11.06.2003. - 14.06.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
NFkB; TFFs gene expression; IkB; experimental colitis

Sažetak
Aims: Trefoil peptides (TFF1, TFF2 and TFF3) are acute phase proteins up-regulated in response to gastrointestinal mucosal damage. They promote cell migration, protect and heal the mucosa and may function as tumorsuppressors. We assumed them to be regulated by the proinflammatory cytokines TNF- , IL1β and IL6, which trigger the transcriptional factors NF-κ B and C/EBPβ . Methods: Gastrointestinal cell lines (KATOIII and HT-29) were stimulated with TNF-a, IL1β , IL6 and the expression of TFFs was monitored by quantitative real-time PCR. In addition TFF3 expression was monitored in TNBS induced colitis rat model. Results: TNF-a, IL1β and IL6 caused a 3- to 11-fold reduction in TFF mRNA expression, displayed in real-time PCR. In vivo epithelial NF-κ B expression coincided with strongly reduced TFF3 expression during the acute phase of experimental colitis. Conclusions: Down-regulation of intestinal trefoil factor TFF3 due to transcriptional repression by TNF-a and IL1β through NF-κ B as well as by IL6 through C/EBPβ activation in vitro may reflect the situation in vivo. This situation may contribute to ulceration and decreased wound healing during inflammatory bowel disease.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA