Naslov
A multiplex PCR assay for the detection of respiratory bacteriae in nasopharyngeal smears from children with acute respiratory disease

Autori
Geertsen, Ralf ; Kaeppeli, Franz ; Sterk-Kuzmanović, Nataša ; Andrašević, Saša ; Anić-Milić, Tatjana ; Dobec, Marinko

Izvornik
Scandinavian Journal of Infectious Diseases (0036-5548) 39 (2007), 9; 769-774

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Multiplex PCR assay; respiratory bacteriae; nasopharyngeal smears; children

Sažetak
To elucidate the frequency of infections with pathogenic respiratory bacteriae during an inter-epidemic period a multiplex PCR assay was used to screen nasopharyngeal smears for the presence of DNA specific for Bordetella pertussis, Bordetella parapertussis, Chlamydophila pneumoniae and Mycoplasma pneumoniae. 187 samples from children aged 2-14 y were analysed with this method in addition to classical bacteriology and compared to results obtained with commercially available PCR kits for each single parameter. From 82 samples positive by bacteriology, 8 (4.3%) were also positive by PCR, whereas from 105 negative samples, 12 (6.4%) were positive only by PCR. From the total of 20 samples positive by PCR, 4 were found to be positive for M. pneumoniae, 6 for B. pertussis, 3 for B. parapertussis and 7 for both B. pertussis and B. parapertussis. Multiplex PCR is a very useful approach for the diagnosis of bacterial infections not detectable by classical bacteriology. In some patients, PCR was the only method giving a positive result, and in others double infections were diagnosed only because of the PCR contribution. Combination of classical bacteriology with multiplex PCR allows a precise diagnosis of infections in the upper respiratory tract, resulting in a more effective therapy.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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