Naslov
Expression of Bcl-2 Family Proteins in Psoriasis

Autori
Batinac, Tanja ; Zamolo, Gordana ; Hadžisejdić, Ita ; Žauhar, Gordana ; Brumini, Gordana ; Ružić, Alen ; Peršić, Viktor

Izvornik
Croatian medical journal (0353-9504) 48 (2007), 3; 319-326

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
apoptosis; psoriasis; bcl-2; skin diseases; apoptosis; proliferation; keratinocytes; bax; epidermis; survival; death; cells

Sažetak
AIM: To elucidate the mechanisms involved in apoptosis of psoriatic keratinocytes by examining the expression of pro-apoptotic (Bak, Bax) and anti-apoptotic (Bcl-2, Bcl-X) Bcl-2 family of proteins, as well as the expression of p53 and Ki-67 proteins in normal skin, and uninvolved and involved psoriatic skin. METHODS: A total of 90 skin samples (30 cases of involved and uninvolved psoriatic skin and normal skin) were examined immunohistochemically to determine the protein expression of p53, Ki-67, Bcl-2, Bcl-X, Bax, and Bak. The results were quantified and expressed as a percentage of positive keratinocytes. RESULTS: There was a significant increase in Ki-67 (17.05 vs 3.65 ; P<0.001), Bcl-X (40.21 vs 13.97 ; P<0.001), Bak (89.46 vs 73.36 ; P<0.001), and Bax (50.00 vs 29.25 ; P<0.001) expression and a decrease in Bcl-2 (3.23 vs 6.25 ; P=0.008) expression in involved psoriatic skin, as well as an increase in Bcl-X (25.13 vs 13.97 ; P<0.001) expression in uninvolved psoriatic skin, when compared to normal skin. Samples with higher percentage of Ki-67 positive cells showed a higher percentage of p53 positive cells (correlation coefficient r=0.75 in involved psoriatic samples, P<0.001 ; r=0.88 in uninvolved psoriatic samples, P<0.001 ; and r=0.85 in normal skin samples, P<0.001). Samples with higher percentage of p53 positive cells expressed pro-apoptotic Bak and Bax in higher percentage of cells ; the correlation coefficients were r=0.74 and r=0.68 in involved psoriatic samples (P<0.001 for both), r=0.75 and r=0.69 in uninvolved psoriatic samples (P<0.001, for both), and r=0.87 and r=0.70 in normal skin samples (P<0.001, for both). CONCLUSION: Increased expression of Bcl-X protein was associated with psoriatic epidermal hyperplasia. Strong Bax and Bak expression in involved psoriatic skin are probably inhibitory mechanisms counteracting intensive proliferation.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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