Naslov
Murine cytomegalovirus clear MHC class I molecules from the cell surface of infected cell and retrieve them in the juxtanuclear pre-late endosomal compartment

Autori
Ilić Tomaš, Maja ; Kučić ; Natalia ; Mahmutefendić, Hana ; Blagojević, Gordana ; Lučin, Pero

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
American Journal of Reproductive Immunology / Kenneth Beaman, Daniel Rukavina - Oxford : Blackwell Publishing, 2007, 430-430

Skup
X International Congress of Reproductive Immunology

Mjesto i datum
Opatija, Hrvatska, 10.06.2007. - 14.06.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Murine cytomegalovirus; MHC class I molecules

Sažetak
Problem. Murine cytomegalovirus (MCMV) downregulate MHC class I molecules (MHC-I) from the cell surface at early stages of infection. The aim of our study was to compare intracellular localization of MHC-I on uninfected and MCMV-infected mouse embrional fibroblasts (MEF). Methods of study. MEFs were infected with deletion mutant of MCMV devoid of viral Fc receptor gene, and intracellular localization and trafficking of MHC-I molecules was determined by confocal microscopy. Cell surface expression of MHC-I molecules was followed by flow-cytometry and intracellular stability by immunoprecipitation after cell surface biotynilation. MHC-I were labeled with monoclonal antibody MA-215 (Kd) and 34-5-8s (Dd). In order to define a compartment wherein MHC-I are accumulated colocalization with a Golgi marker (GM130), an early endosome marker (EEA-1), a lysosomal marker (LAMP-1) and with antibody against TfR (R-17) were used. Microtubular network was disrupted by nocodazole (6.6 μ M). Results. We have found that Kd and Dd molecules, which are present at the surface of uninfected cells, are down-regulated from the surface of infected cells. These molecules can be found accumulated in juxtanuclear region of infected cells in large vesiculo-tubular structures. Kd and Dd molecules colocalized with GM130, R-17 and in small amount with LAMP-1 and EEA-1. In order to dissect whether these molecules are retrieved by the Golgi or in endosomal vesicles, we used nocodazole to disperse juxtanuclear organelle, including the Golgi complex. After nocodazole treatment, Golgi complex was dispersed into vesicles containing Kd and Dd molecules, however almost half of retained molecules did not colocalize with GM130. Conclusions. From these results we can conclude that virus down-regulates the MHC-I from the cell surface of infected cells into the juxtanuclear vesicles that are after sorting endosomes in the endocytic pathway (EEA-1) and before late endosomes (Lamp1). These vesicles are outside of the recycling route (TfR-). Significant amount of the nascent MHC-I molecules are, in addition, retained in the Golgi complex. Supported by Croatian Ministry of Science, Grant No 0620238-0223.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA