Naslov
Prognostic significance of intratumoral heterogeneity for cathepsin D and cyclin D1 expression in laryngeal squamous cell carcinoma

Autori
Ćorić, Marijana ; Batelja, Lovorka ; Bumber, Željko ; Đanić, Davor ; Seiwerth, Sven

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 20th Europan Congress of Pathology ; u: Virchows Archiv 447 (2005) (2) / - Berlin : Heidelberg : Springer, 2005, 480-480

Skup
Europan Congress of Pathology (20 ; 2005)

Mjesto i datum
Pariz, Francuska, 03.09.2005. - 08.09.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
laryngeal squamous cell carcinoma; cathepsin D; cyclin D1
(aryngeal squamous cell carcinoma; cathepsin D; cyclin D1)

Sažetak
Intratumoral heterogeneity is defined as the parallel existence of different (e.g.diploid and aneuploid)clones within a given tumor.This implies that different tumor parts could express different behavior (e.g.propensity to invade or metastasize, resistance against chemo-or radiotherapy).Current investigations mostly neglect intratumoral heterogeneity in general and completely ignore the possibility of regional intratumoral heterogeneity.The goals of this investigation were to define intratumoral heterogeneity of cyclin D1, cathepsin D and nm23 protein as well as nm23H1 gene in three different tumor regions (tumor center, invasive margin and transformation region), to establish potential prognostic significance of regional factors and to establish potential correlation between the investigated factors and classical prognostic factors (T, N, M, histological grade). We performed the study on a group of 170 patients with laryngeal cancer undergoing partial or complete laryngectomy.After standard paraffin embedding immunohistochemistry was performed using manufacturers protocols (DAKO, Oncogene Sci.).Immunoreactivity was accessed using semiquantitative score.Follow up of patients was minimum 60 months for survivors.Statistics was performed using Statistica for Windows and RPART statistics. Results of our study clearly show that the intratumoral heterogeneity is not a random event, but that there is a clear areal distribution of immunoreactivity to cathepsin D and cyclin D1, with the invasive margin expressing the strongest immunoreactivity followed by the tumor center.There is positive correlation for expression of cathepsin D in tumor cells with T (p=0, 003), and negative correlation for stromal expression of cathepsin D with T (p=0, 006), N (p=0, 01)and histological grade (p=0, 02).Areal expression of cathepsin D and cyclin D1 showed prognostic significance on Kaplan- Meier curves (cathepsin D (p=0, 001)cyclin D1 (p=0, 008)). Using RPART statistics the expression of cathepsin D in tumor stromal cells of tumor center was demonstrated as being a classificator equal to standard prognostic parameters. These findings strengthen the concept that areal intratumoral heterogeneity should not be neglected neither in research nor in diagnostic work.

Izvorni jezik
Engleski

Napomena
DOI: 10.1007/s00428-005-1288-1

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