Naslov
Molecular typing of Plum pox virus isolates in Croatia

Autori
Kajić, Vesna ; Černi, Silvija ; Krajačić, Mladen ; Mikec, Ivan ; Škorić, Dijana

Izvornik
Journal of Plant Pathology (1125-4653) 90 (2008), 1, Supplement S; 9-13

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
PPV-Rec; (Cter)CP; (Cter)NIb/(Nter)CP; phylogeny; molecular diversity; strain typing

Sažetak
Previous studies demonstrated the presence of PPV-M and PPV-D strains in various Prunus species all over Croatia. Susceptible plum varieties, known as particularly endangered by recombinant PPV (PPV-Rec), were in the focus of molecular typing encompassed by this study. Samples were collected in commercial orchards raised with imported plant material, except for one taken from the urban Zagreb area. Six out of seven samples tested PPV positive by DAS-ELISA. A modified IC-RT-PCR approach was carried out in order to amplify two target sequences, the carboxy-terminus part of capsid cistron ((Cter)CP) and the virus segment spanning the carboxy-terminus part of NIb ((Cter)NIb) to the amino-terminus part of CP ((Nter)CP). The PCR strain identification based on the use of the primers targeting (Cter)CP region revealed the presence of PPV-M in all positive samples. The presence of PPV-D was found in 3 samples in mixed infection with PPV-M. Two samples harbored PPV-Rec (‘ Valjevka’ , wild plum). Six sequences of (Cter)CP amplicons clustered in two groups, in accordance with the strain differentiation (PPV-M, -D), while two (Cter)NIb/(Nter)CP sequences clustered within the group of well known PPV-Rec isolates. Phylogenetic analyses suggest different origin of two PPV-Rec isolates in accordance with biological data. If distinct geographical position and different source plant genesis are considered, higher molecular diversity of PPV in Croatia than previously reported is predictable from this first PPV-Rec report.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

POVEZANOST RADA