Naslov
Development and prevalidation of a method for phenol determination by solid-phase spectrophotometry

Autori
Vuković, Jadranka ; Matsuoka, Shiro ; Yoshimura, Kazuhisa ; Grdinić, Vladimir ; Jurišić Grubešić, Renata

Izvornik
Microchimica Acta (0026-3672) 159 (2007), 3-4; 277-285

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
phenol determination; prevalidation strategy; solid-phase spectrophotometry; 4-aminoantipyrine; pharmaceutical application

Sažetak
A simple, sensitive and rapid solid-phase spectrophotometric procedure was developed for the determination of traces of phenol with 4-aminoantipyrine as a reagent (AAP-SPS), and the optimal experimental conditions were established. This method was performed by sorption and direct absorbance measurements of the product phenol-AAP sorbed on the anion-exchanger Dowex 1-X4 (0.2 g) at 495 nm (absorption maximum) and 700 nm (non-absorption wavelength). The sensitivity offered by the AAP-SPS procedure was higher by a factor of 40 compared with the respective conventional spectrophotometric method. Metrological characteristics were established using a prevalidation strategy. The AAP-SPS procedure is characterized by a linear calibration function in the working range of 0.05-0.50 mu mol, low standard deviation of procedure (+/- 0.012), low limit of determination (0.021 mu mol), and favorable random (+/- 0.85 to +/- 11.27%) and systematic deviations (-4.55 to +/- 11.50%). Moreover, the accuracy of the system investigated by the recovery test is acceptable (99-102%). Favorable working and performance characteristics make the new SPS method ideal for phenol monitoring in pharmaceutical preparations as well as other matrices.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA