Naslov
Capillary Electrophoresis Determination of Pravastatin and Separation of Its Degradation Products

Autori
Nigović, Biljana ; Vegar, Ivana

Izvornik
Croatica Chemica Acta (0011-1643) 81 (2008), 4; 615-622

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
pravastatin; capillary zone electrophoresis; pharmaceuticals; micellar electrokinetic chromatography; degradation products

Sažetak
A capillary zone electrophoresis method for pravastatin determination was developed and validated. A rapid migration of negatively charged pravastatin molecule was obtained under alkaline buffer and applied electric field of 30 kV. The influence of the pH value and ionic strength of running buffer, applied voltage and capillary temperature on mobility and sensitivity was evaluated. The detection wavelength was set to 237 nm. The method was applied to the determination of the drug in pharmaceutical dosage form. Pravastatin is a  -hydroxy acid which is prone to lactonize and epimerize in a pH-dependent manner. Micellar electrokinetic chromatographic approach was chosen to develop a method able to separate pravastatin and its degradation products in acidic media. Proposed method allows baseline separation of hydroxy acid and neutral lactone forms of the drug which appear as interconversion products depending on pH value.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA