Pregled bibliografske jedinice broj: 33140
Ribosomal RNA genes in Quercus; copy number, interphase and chromosomal organization and expression
Ribosomal RNA genes in Quercus; copy number, interphase and chromosomal organization and expression // Periodicum BIologorum, Century of continuity 100(1) / Vitale, Branko (ur.).
Zagreb, 1998. (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 33140 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Ribosomal RNA genes in Quercus; copy number, interphase and chromosomal organization and expression
Autori
Zoldoš, Vlatka ; Papeš, Dražena ; Cerbah, Malika ; Panaud, Olivier ; Šiljak-Yakovlev, Sonja
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Periodicum BIologorum, Century of continuity 100(1)
/ Vitale, Branko - Zagreb, 1998
Skup
First Congress of Croatian Geneticists with International Participation
Mjesto i datum
Hvar, Hrvatska, 01.06.1998. - 04.06.1998
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
Quercus; fluorescent in situ hybridization; rRNA genes
Sažetak
Two classes of ribosomal gene families, 5S rRNA and 18S-26S rRNA, were physically mapper on the oak metaphase chromosomes and interphase chromatine using fluorescent in situ hybridization. All the species investigated revealed the same FISH pattern i.e. the same number of 5S and 18S-26S rDNA loci and their chromosomal position. They had one major terminal locus and two paracentromeric minor 18S-26S loci and one paracentromeric 5S rDNA locus. Simultaneously hybridzation experiment with two differentially labelled probes showed that three 18S-26S rDNA loci a separated on the different chromosomes, thoughly one minor 18S-26S rDNA and 5S rDNA locus were co-located. FISH analyses of interphase nuclei revealed the spatial arrangement of rDNA loci and suggested expression patterns. 18S-26S rDNA frequently exhibited "gene-puffs"as decondensed chromatine strings within the nucleoli. Decondensation and spatial proxinity of rDNA clusters to the nucleolus indicated their activity. Abundant punctuate hybridisation signals present around the nucleoli visible even in the metaphase indicated the late transcriptional activity of the genes of the main 18S-26S locus. As the variation of these locus appeared in the strength and the size of the hybridisation signal, both between and within species, the overal copy number of these genes were estimated by slot-blotting. Inter- and intra-specific differences were revealed.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
