Pregled bibliografske jedinice broj: 33056
RAPD analyses, genome size and genomic in situ hybridization in triploid viviparous onions
RAPD analyses, genome size and genomic in situ hybridization in triploid viviparous onions // Periodicum Biologorum, Century of continuity, 100(1) / Vitale, Branko (ur.).
Zagreb, 1998. (poster, domaća recenzija, sažetak, znanstveni)
CROSBI ID: 33056 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
RAPD analyses, genome size and genomic in situ hybridization in triploid viviparous onions
Autori
Puizina, Jasna ; Javornik, Branka ; Bohanec, Borut ; Klein, Franz ; Schweizer, Franz ; Schweizer, Dieter ; Maluszynska, Jolanta ; Papeš, Dražena
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Periodicum Biologorum, Century of continuity, 100(1)
/ Vitale, Branko - Zagreb, 1998
Skup
First Congress of Croatian Geneticists with International Participation
Mjesto i datum
Hvar, Hrvatska, 01.06.1998. - 04.06.1998
Vrsta sudjelovanja
Poster
Vrsta recenzije
Domaća recenzija
Ključne riječi
Allium cepa var. viviparum Metzg. (Alef.) auct.; genomic fluorescent in situ hybridization; RAPD analyses; genome size
Sažetak
Identical Randomly Amplified Polymorphic DNA (RAPD) banding pattern and the same DNA amount (2C=43,4 pg) confirmed the high genetic similarity and the unique origin of the two triploid viviparous onions (Allium cepa var. viviparum Metzg. (Alef.) auct.), the Croatian Ljutika and the Indian Pran. In order to elucidate the parental Allium species of these natural triploid hybrids, genomic fluorescent in situ hybridization was applied. Biotynilated genomic DNAs from six diploid Allium species (A. cepa L., A. fistulosu, L., A. roylei Stearn, A. vavilovii M. Pop et Vved., A. galanthum Kar. et Kir., A. oschaninii O. Fedtsch.) were used as probes in this study. The DNA probe from A. cepa labelled compeletely only one, C genome, (8 longer chromosomes). The DNA probe from A. roylei labelled completely another, R genome (8 rather short chromosomes), and one or both telomeric regions of at least 10 other chromosomes. A few chromosomes, the markers of the triploid caryotype were not completely labelled neither with A. cepa DNA probe, nor with A. roylei DNA probe. The results unambiguously showed identity of two parental genomes (C and R), whereas the third genome (X) still need to be identified.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
