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Pregled bibliografske jedinice broj: 329331

Separation of plant viral satellite double-stranded RNA using high-performance liquid chromatography

Likić, Saša; Rusak, Gordana; Krajačić, Mladen
Separation of plant viral satellite double-stranded RNA using high-performance liquid chromatography // Journal of Chromatography A, 1189 (2008), 1-2; 451-455 doi:10.1016/j.chroma.2007.12.074 (međunarodna recenzija, članak, znanstveni)

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Naslov
Separation of plant viral satellite double-stranded RNA using high-performance liquid chromatography

Autori
Likić, Saša ; Rusak, Gordana ; Krajačić, Mladen

Izvornik
Journal of Chromatography A (0021-9673) 1189 (2008), 1-2; 451-455

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
cucumber mosaic virus; satRNA; HPLC; detection; purification

Sažetak
High-performance liquid chromatography was developed for further separation of double-stranded (ds) RNAs obtained by CF-11 cellulose chromatography from plants infected with satellite associated cucumber mosaic virus. Fractions separated by monolithic polymer column, especially applicable for nucleic acid analyses, were identified electrophoretically and confirmed with a polymerase chain reaction test. Once standardized, the method has revealed clear evidence of satellite presence without precipitation and electrophoresis. According to demonstrated sensitivity, its application in the preliminary diagnostics of field samples is also predictable. Principally, it can be used as a powerful preparative approach resulting in highly pure satellite dsRNA for further analyses.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA

Projekti:
119-1191192-1213 - Flavonoidi i molekularni mehanizmi njihovih bioloških učinaka (Rusak, Gordana, MZOS ) ( CroRIS)
119-1191192-1222 - Molekularna varijabilnost biljnih patogena (Krajačić, Mladen, MZOS ) ( CroRIS)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Gordana Rusak (autor)

Avatar Url Saša Likić (autor)

Avatar Url Mladen Krajačić (autor)

Poveznice na cjeloviti tekst rada:

doi www.sciencedirect.com

Citiraj ovu publikaciju:

Likić, Saša; Rusak, Gordana; Krajačić, Mladen
Separation of plant viral satellite double-stranded RNA using high-performance liquid chromatography // Journal of Chromatography A, 1189 (2008), 1-2; 451-455 doi:10.1016/j.chroma.2007.12.074 (međunarodna recenzija, članak, znanstveni)
Likić, S., Rusak, G. & Krajačić, M. (2008) Separation of plant viral satellite double-stranded RNA using high-performance liquid chromatography. Journal of Chromatography A, 1189 (1-2), 451-455 doi:10.1016/j.chroma.2007.12.074.
@article{article, author = {Liki\'{c}, Sa\v{s}a and Rusak, Gordana and Kraja\v{c}i\'{c}, Mladen}, year = {2008}, pages = {451-455}, DOI = {10.1016/j.chroma.2007.12.074}, keywords = {cucumber mosaic virus, satRNA, HPLC, detection, purification}, journal = {Journal of Chromatography A}, doi = {10.1016/j.chroma.2007.12.074}, volume = {1189}, number = {1-2}, issn = {0021-9673}, title = {Separation of plant viral satellite double-stranded RNA using high-performance liquid chromatography}, keyword = {cucumber mosaic virus, satRNA, HPLC, detection, purification} }
@article{article, author = {Liki\'{c}, Sa\v{s}a and Rusak, Gordana and Kraja\v{c}i\'{c}, Mladen}, year = {2008}, pages = {451-455}, DOI = {10.1016/j.chroma.2007.12.074}, keywords = {cucumber mosaic virus, satRNA, HPLC, detection, purification}, journal = {Journal of Chromatography A}, doi = {10.1016/j.chroma.2007.12.074}, volume = {1189}, number = {1-2}, issn = {0021-9673}, title = {Separation of plant viral satellite double-stranded RNA using high-performance liquid chromatography}, keyword = {cucumber mosaic virus, satRNA, HPLC, detection, purification} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

Uključenost u ostale bibliografske baze podataka::


  • Analytical Abstracts
  • MEDLINE

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