Naslov
Effect of silicone based endodontic material on cytokine production by peritoneal mice macrophage

Autori
Peršić, Romana ; Gazdik, Hrvoje ; Miletić, Ivana ; Brekalo Pršo, Ivana ; Kuiš, Davor ; Pezelj-Ribarić, Sonja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
Stomatologija - svakodnevna praksa : Kongres s međunarodnim sudjelovanjem

Mjesto i datum
Zagreb, Hrvatska, 14.03.2008. - 15.03.2008

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
silicone based endodontic sealer

Sažetak
The aim of this study was to determine the in vitro effect of RoekoSeal (RSA), silicone-based endodontic sealer, on cytokine IL-1 and IL-6 production in mice macrophages. Freshly mixed sample of RoekoSeal was placed in contact with cultured mice macrophages. The supernatant from the cultures was assayed for IL-1 and IL-6 cytokine using enzyme-linked immunosorbent assay. Cells cultured in the absence of tested materials served as negative controls. As a positive control, similar cultures were also exposed to lipopolysaccharide. The silicone based material examined in these experiments did not stimulate increased IL-1 and IL-6 release from cell lines. The difference between IL -1 production by macrophages exposed to the tested materials and IL-6 production by the same macrophages was tested by one-way ANOVA. To compare LPS stimulated IL -1 and IL -6 production in the positive control group and to compare IL-1 and IL-6 production in negative control group were used Man-Whitney test. The silicone based material did not stimulate increased IL-1 and IL -6 release from cell lines examined.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

POVEZANOST RADA