Naslov
Inhibition of antigen-induced degranulation and IL-4 production from RBL-2H3 cells by Lavandula hybrida and L. angustifolia extracts

Autori
Blažeković, Biljana ; Marković, Stribor ; Vladimir-Knežević, Sanda

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Planta Medica 2007: 55th International Congress and Annual Meeting of the Society for Medicinal Plant Research. 73, 797-1034. / Bucar, Franc ; Bauer, Rudolf - Stuttgart : Georg Thieme Verlag, 2007, 841-841

Skup
55th International Congress and Annual Meeting of the Society for Medicinal Plant Research

Mjesto i datum
Graz, Austrija, 02.09.2007. - 06.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Lavandula hybrida; Lavandula angustifolia; antiallergic activity; IL-4

Sažetak
Allergic diseases are considered to be multifactorial inflammatory diseases that present a serious health problem worldwide while their prevalence have continuously increased during the past few decades. They are results of type I hypersensitivity mediated through the effects of antigen-specific IgE which binds to mast cells and, on subsequent cross-linking by allergen reexposure, causes cell degranulation with the release of preformed mediators in the early-phase as well as the production of cytokines in the late-phase. The aim of our study was to investigate the antiallergic activities of cultivated Lavandula hybrida Rev. and L. angustifolia P. Mill. using the rat basophilic leukaemia cell line (RBL-2H3), a well-characterised in vitro model of mast cells. The ethanolic extracts of flowers of L. hybrida (LHE) and L. angustifolia (LAE) were found to significantly inhibit release of β -hexosaminidase, the marker of antigen-IgE-mediated degranulation in RBL-2H3 cells, showing similar activities (IC50=37 ug/mL and IC50=42 ug/mL, respectively). Furthermore, using the enzyme-linked immunosorbent assay (ELISA), we established that LHE and LAE inhibit antigen-induced production of anti-inflammatory cytokine IL-4 (IC50=60 ug/mL and IC50=45 ug/mL, respectively). Among ten investigated principal bioactive compounds, luteolin, apigenin and ursolic acid were found to be the most potent inhibitors of degranulation as well as cytokine production. In conclusion, the tested extracts showed antiallergic effects acting on the both phases of type I reaction. Therefore, our results suggest a possible therapeutic application of Lavandula species in allergy and allergy-related diseases.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA