Naslov
Flavonoid Content Assay: Prevalidation and Application on Plantago L. Species

Autori
Jurišić Grubešić, Renata ; Vuković, Jadranka ; Kremer, Dario ; Vladimir-Knežević, Sanda

Izvornik
Acta Chimica Slovenica (1318-0207) 54 (2007), 2; 397-406

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
prevalidation strategy; flavonoids; aluminium chloride; Plantago L.

Sažetak
This work is aimed to prevalidate and apply UV/Vis spectrophotometric procedure for flavonoid determination in herbal material using aluminium chloride as a reagent. Fast and simple, full prevalidation for quality contol and standardization od analytical procedure, based on mathematical/statistical testing coupled with system of diagnosis was used to evaluate and demonstrate the reliability of method for flavonoid determination with aluminium chloride (F-Al procedure). Favourable prevalidation characteristics verified this procedure as a valuable tool in flavonoid analysis, so it was successfully applied for determination of flavonoids in leaves, stems and flowers of Plantago L. species growing in Croatia. The results showed the various flavonoid contents between different plant parts (leaves: up to 0.13% ; stems: up to 0.07% and flowers: up to 0.07%).The results of flavonoid determination were statistically evaluated by using Principal Component Analysis (PCA) and Student's t-test as a contribution to investigation of different taxa of genus Plantago L.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

POVEZANOST RADA