Naslov
Preprotein recognition by the Toc complex

Autori
Becker, Thomas ; Jelić, Marko ; Vojta, Aleksandar ; Radunz, Alfons ; Soll, Jürgen ; Schleiff, Enrico

Izvornik
EMBO Journal (0261-4189) 23 (2004), 3; 520-530

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Preprotein recognition; Toc complex; Toc159

Sažetak
The Toc core complex consists of the pore-forming Toc75 and the GTPases Toc159 and Toc34. We confirm that the receptor form of Toc159 is integrated into the membrane. The association of Toc34 to Toc75/Toc159 is GTP dependent and enhanced by preprotein interaction. The N-terminal half of the pSSU transit peptide interacts with high affinity with Toc159, whereas the C-terminal part stimulates its GTP hydrolysis. The phosphorylated C-terminal peptide of pSSU interacts strongly with Toc34 and therefore inhibits binding and translocation of pSSU into Toc proteoliposomes. In contrast, Toc159 recognises only the dephosphorylated forms. The N-terminal part of the pSSU presequence does not influence binding to the Toc complex, but is able to block import into proteoliposomes through its interaction with Toc159. We developed a model of differential presequence recognition by Toc34 and Toc159.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA