Naslov
Pomaceous fruit tree phytoplasmas and their potential vectors in Croatia

Autori
Križanac, Ivana ; Mikec, Ivan ; Budinščak, Željko ; Šeruga Musić, Martina ; Krajačić, Mladen ; Škorić, Dijana

Izvornik
Acta Horticulturae (0567-7572) 781 (2008); 477-482

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
pear decline; psyllids; 16S rDNA; PCR; RFLP

Sažetak
Surveys of pomaceous fruit trees were carried out in the years 2003 and 2004 in order to determine the current status of ‘ Candidatus Phytoplasma pyri’ (pear decline, PD) and ‘ Ca. P. mali’ (apple proliferation, AP) in Croatia. PD and AP were in the focus of our interest because they are on the Croatian A2 quarantine list of pests and diseases. Eleven orchards were visually inspected for the symptom presence during summer and autumn. Leaf and twig samples were collected from symptomatic pear and apple trees only. In 2004 potential insects vectors were collected from 5 orchards. Extracted nucleic acids were PCR-tested using primers amplifying 16S rDNA. Digested amplicons showed restriction profiles referable to phytoplasmas belonging to one of the ribosomal subgroups 16SrX-C, 16SrXII-A, 16SrI-B, recently designated as ‘ Ca. P. pyri’ , ‘ Ca. P. solani’ and ‘ Ca. P. asteris’ , respectively. Mixed infections were detected in one pear and one insect sample. No molecular evidence for the presence of ‘ Ca. P. mali’ in Croatia was found within the scope of this research.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

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