Naslov
Ultrasructure of the notochordal sheath in Branchiostoma lanceolatum L.

Autori
Bočina, Ivana ; Saraga-Babić, Mirna

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Dumić, Jerka - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2002, 129-129

ISBN
953-6256-13-4

Skup
1st Croatian congress on molecular life sciences

Mjesto i datum
Opatija, Hrvatska, 09.06.2002. - 13.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
amphioxus ; notochord ; ultrastructure

Sažetak
The notochordal sheath of the lancelet Branchiostoma lanceolatum L. was investigated in ten adult specimens by electron microscopy. The specimens of tissue were prepared for electron microscope analysis by fixing them in 3, 5% paraformaldehyde and in 3% glutaraldehyde in PBS. The postfixation was done in 2% osmium tetroxide. The tissue was embeded in Epoxy resin. The ultrathin sections of tissue were stained with uranyl acetate and lead citrate. the notochordal sheath bounds the notochord from all sides without interruptions. Ultrastructurally, the notochordal sheath shows three.layered organization. In cross section the outer layer consists of thick and thin collagen fibers. In the middle layer collagen fibers are first oblique and then longitudinal, what indicates their spiral course. The collagen fibers are not of the same thickness, but they are all cross-striated. Some fibers are thick and straight, and they correspond to collagen type I in vertebrates. The others are thin and branched and they probably correspond to collagen type III (reticulin fibers). The inner layer is the thinnest layer of the sheath. It is amorphous and granular in structure(like basal lamina)and it is closely attached to the notochord by hemidesmosome-like junctions. Just beneath the inner layer of the sheath lays the peripheral part of the notochord, made of Muller's tissue. The cytoplasm of Muller's cells is bound to the inner layer of the sheath by hemidesmosome-like junctions.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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