Naslov
Automated quantification of IL-1alfa mRNA by GeneScan analysis software

Autori
Forčić, Dubravko ; Košutić, Tanja ; Branović, Karmen ; Mažuran, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Journal of Molecular Medicine / Ganten D. - Berlin : Springer, 1997, B54-B54

ISBN
0946-2716

Skup
Congres of Molecular Medicine

Mjesto i datum
Berlin, Njemačka, 03.05.1997. - 05.05.1997

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
mRNA; IL1; GeneScan; quantification

Sažetak
The measurement of a cytokine expression during an immune response is very important for studying basic immune mechanisms and the pathogenesis of a disease. The analysis of cytokine mRNA may be an appropriate measurement for cytokine protein levels, because the production of many cytokines is primarily transcriptionally regulated. We have used the automated method for quantification of IL-1a mRNA by Gene Scan analysis software (Perkin Elmer), synthetic pAW109 RNA (internal standard) and GeneScan-500 TAMRA (size standard) for PCR analysis of cytokine production in human monocytes after appropriate stimulation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA