Naslov
Development and validation of the HPLC method for determination of nucleotides in infant formulas

Autori
Krpan, Marina ; Hruškar, Mirjana ; Marković, Ksenija ; Vahčić, Nada

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Annals of Nutrition & Metabolism / Guilloteau, Paul ; Lemonnier, Daniel ; Lairon, Denis - Basel : Karger Publishers, 2007, 170-171

ISBN
978-3-8055-8335-0

Skup
10th European Nutrition Conference

Mjesto i datum
Pariz, Francuska, 10.07.2007. - 13.07.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
HPLC method; Infant formulas; Nucleotides; Validation
(HPLC method; infant formulas; nucleotides; validation)

Sažetak
Researchers have established that human milk is the only source of nucleotides for infant during first months of life. Dietary nucleotides seem to influence several aspects of neonatal development such as modulate lipoprotein metabolism, modify the composition of the intestinal microflora, improve gastrointestinal tract, repair after damage and participate in the immunity response mediated by T-cells. Since, the infant formulas supplemented with nucleotides are developed. The aim of these investigations was development of the method for determination of 5’ -AMP, 5’ -CMP, 5’ -GMP, 5’ -UMP and 5’ -IMP in infant formulas. Because the chosen methods can influence on the quality of achieved results, in this work the validation of HPLC methods has been studied. A method for the determination of 5’ -AMP, 5’ -CMP, 5’ -GMP, 5’ -UMP and 5’ -IMP in infant formulas was optimized and its performance evaluated. Method is based on deproteinisation of samples and direct analysis by ion-pair HPLC using Supelcolsil LC-18-T column, followed by diode-array detection. During validation process the following parameters were examined: selectivity, precision, accuracy, linearity and robust. The obtained results are summarised and showed that this method is specificity. The precision of the method is evaluated and a coefficient of variation (CV %) is less than 3 %. Validation of the proposed method was carried out by standard additions method, with recoveries of 100 ± 3 ; the relative standard deviation was ≤ 3. For linearity the correlation coefficient was r2 ≥ 0, 999. Solutions are stable during 30 hours and in range of method robustness. Validation of HPLC method for determination of nucleotides has showed that developed analytical method is acceptable for its intended purpose in defined labour conditions.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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