Naslov
Cellular uptake and cytotoxicity in vitro and toxicity in vivo of DNA and RNA intercalator ADAP

Autori
Marczi, Saška ; Stojković, Ranko ; Belovari, Tatjana ; Šerić, Vatroslav ; Piantanida, Ivo ; Glavaš-Obrovac, Ljubica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XXXVI Annual Meeting of the SBBq and 10th IUBMB Conference "Infectious Diseases: Biochemistry of Parasites, Vectors and Hosts", Program and Abstracts / - , 2007

Skup
XXXVI Annual Meeting of the SBBq and 10th IUBMB Conference "Infectious Diseases: Biochemistry of Parasites, Vectors and Hosts"

Mjesto i datum
Salvador, Brazil, 21.05.2007. - 25.05.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
4; 9-diazapyrenium; DNA intercalation; uptake; cytotoxicity; toxicity

Sažetak
CELLULAR UPTAKE AND CYTOTOXICITY IN VITRO AND TOXICITY IN VIVO OF DNA AND RNA INTERCALATOR ADAP Marczi, S.1 ; Stojković, R.2 ; Belovari, T.3 ; Šerić, V.1 ; Piantanida, I.2 ; Glavaš-Obrovac, Lj.1, 3 1Clinical Hospital Osijek ; 2Ruđer Bošković Institute, Zagreb ; 3School of Medicine Osijek, Osijek, Croatia Introduction: 4-Metyl-2, 7-diamino-5, 10-diphenyl-4, 9-diazapyrenium hydrogensulfate (ADAP) is a potential antitumor and antiparasitic compound because of its DNA and RNA intercalating ability. The objectives of this study were to monitor uptake and intracellular distribution of ADAP, to investigate cytotoxic effects on human normal cells and various human, and mouse tumor cell lines, as well as to examine toxicity of ADAP on mouse model. Results: The ADAP entered into MIAPaCa-2 cell’ s cytoplasm in five minutes and into nuclei in sixty minutes after administration. MTT test showed that ADAP (0.1 – 100 μ M) strongly inhibited growth of both mouse (FsaR, SCCVII) and human tumor cells (HeLa, Caco-2, HT-29, MIAPaCa-2, HBL, HEp-2, SW620, MCF-7) compared to its weak cytotoxic effects on controls and normal cells (WI38). Toxic effects of single and multiple LD10 doses of ADAP were not detected in treated mice (C3Hf/BuZGr) using hematological and clinical-chemical analysis of blood and histopathological examinations. Conclusions: Obtained findings indicate antitumor activity of ADAP. Based on DNA intercalating feature of this compound and due to absence of of its toxic effects on mice, we intend to examine antiparasitic activity of ADAP.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti

POVEZANOST RADA