Naslov
Meningiomas exhibit loss of heterozygosity of the APC gene

Autori
Nikuševa Martić, Tamara ; Pećina-Šlaus, Nives ; Beroš, Vili ; Tomas, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Oncogene and Human cancer : The next 25 Years : Abstracts / Donate, Luis - Madrid : CNIO Centro Nacional de Investigaciones Oncologicas, 2007

Skup
Oncogene and Human cancer : The next 25 Years

Mjesto i datum
Madrid, Španjolska, 03.10.2007. - 06.10.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
adenomatous polyposis coli gene (APC); loss of heterozygosity; meningiomas; tumors of the CNS; wnt signaling pathway

Sažetak
The molecular mechanisms and candidate genes involved in development of meningiomas still need investigation and elucidation. In the present study thirty-three meningiomas were analyzed regarding genetic changes of tumor suppressor gene Adenomatous polyposis coli (APC), a component of the wnt signalling. Gene instability was tested by polymerase chain reaction/loss of heterozygosity (LOH) using Restriction Fragment Length Polymorphism (RFLP) method. RFLP was performed by two genetic markers, Rsa I in APC’ s exon 11 and Msp I in its exon 15. The results of our analysis showed altogether 15 samples with LOH of the APC gene out of 32 heterozygous patients (47%). Seven patients had LOHs at both exons, while 4 LOHs were exclusive for exon 11 and 4 for exon 15. The changes were distributed according to pathohistological grade as follows: 46% of meningothelial meningioma showed LOH ; 33% of fibrous ; 75% of mixed (transitional) ; 75% of angiomatous, and one LOH was found in a single case of psammomatous meningioma. None of the LOHs were found in atypical and anaplastic cases. The results of this investigation suggest that genetic changes of APC gene, play a role in benign meningioma formation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA