Naslov
Changes of serum glycans during sepsis and acute pancreatitis

Autori
Gornik, Olga ; Royle, Louise ; Harvey, David J. ; Radcliffe, Catherine M. ; Saldova, Radka ; Dwek, Raymond A. ; Rudd, Pauline ; Lauc, Gordan

Izvornik
Glycobiology (0959-6658) 17 (2007), 12; 1321-1332

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
serum glycan structures; glycosylation; sepsis; pancreatitis

Sažetak
Acute inflammatory response is a complex process associated with production of both pro- and antiinflammatory mediators. Although it is generally considered to be a single homeostatic mechanism, there are differences associated with the nature and the site of inflammation. We examined changes of N-linked glycans released from serum of a patient with sepsis and a patient with acute pancreatitis during first eight days of disease. Sera were taken from patients at the time of reporting to hospital and then three more times. The blood from a healthy individual was drawn on one occasion only. Glycans were released using N-glycosidase F and subjected to normal phase and weak anion exchange HPLC, exoglycosidase digestions and mass spectrometry. Levels of identified structures have been followed through the course of disease and compared to the control levels. Changes in serum glycans were found to occur very early in acute inflammation. Most prominent differences include the increase in ratio of outer arm to core fucose, increase in the amount of tetrasialylated structures, changes in levels of mannose structures and in the degree of branching. The relative proportions of different glycans changed daily and some differences were also observed between sepsis and pancreatitis, probably reflecting that in these two conditions the acute phase response is triggered by a different stimulus which is associated with different patterns of production of cytokines.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti

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