The role of fat dormouse (Glis glis L.) as reservoir host for spirochete Borrelia burgdorferi sensu lato in the region of Gorski Kotar, Croatia

Turk, Nenad; Milas, Zoran; Margaletić, Josip; Turk, Romana; Barbić, Ljubo; Konjević, Dean; Perić, Sanja; Štritof, Zrinka; Starešina, Vilim

doi:10.1007/s10344-007-0119-x

Pregled bibliografske jedinice broj: 318749

The role of fat dormouse (Glis glis L.) as reservoir host for spirochete Borrelia burgdorferi sensu lato in the region of Gorski Kotar, Croatia

Turk, Nenad; Milas, Zoran; Margaletić, Josip; Turk, Romana; Barbić, Ljubo; Konjević, Dean; Perić, Sanja; Štritof, Zrinka; Starešina, Vilim

The role of fat dormouse (Glis glis L.) as reservoir host for spirochete Borrelia burgdorferi sensu lato in the region of Gorski Kotar, Croatia // European journal of wildlife research, 54 (2008), 1; 117-121 doi:10.1007/s10344-007-0119-x (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 318749 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The role of fat dormouse (Glis glis L.) as reservoir host for spirochete Borrelia burgdorferi sensu lato in the region of Gorski Kotar, Croatia

Autori
Turk, Nenad ; Milas, Zoran ; Margaletić, Josip ; Turk, Romana ; Barbić, Ljubo ; Konjević, Dean ; Perić, Sanja ; Štritof, Zrinka ; Starešina, Vilim

Izvornik
European journal of wildlife research (1612-4642) 54 (2008), 1; 117-121

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
fat dormouse; glis glis; Lyme borreliosis; Borrelia burgdorferi sensu lato; Borrelia afzelii; PCR-RFLP

Sažetak
To determine whether some of the B. burgdorferi sensu lato genospecies associate with fat dormouse as a reservoir host, we investigated the prevalence of infection in questing animals. A total of 45 adult fat dormice (30 female and 15 male) were captured by hunters during their hunting season in the region of Gorski Kotar, Croatia. Dead animals were aseptically dissected and the urinary bladder tissue was used for isolation attempt and for the DNA extraction. Out of 45 DNA samples extracted from urine bladder tissue we found 4 (8.88%) to be PCR positive. The RFLP analysis of the PCR product after cleavage with DraI and MseI distinguished between the three major genospecies: B. burgdorferi sensu stricto, B. garinii and B. afzelii. All positive samples were typed as B. afzelii with a unique DraI or MseI pattern. The results of the analysis of urinary bladder tissue samples culture for the presence of Borrelia were negative. Results showed a prevalence of the Borrelia infection among population of fat dormice indicated their epizootiological involvement as a reservoir of Borrelia spirochetes. Furthermore, this work is an initial step in the investigation of the molecular epidemiology/epizootiology of Lyme borreliosis in Croatia.

Izvorni jezik
Engleski

Znanstvena područja
Veterinarska medicina, Šumarstvo

POVEZANOST RADA

Projekti:
024-0682041-2098 - Očuvanje stabilnosti i produktivne sposobnosti šumskih kultura (Perić, Sanja, MZOS ) ( CroRIS)
053-0481153-1129 - Mikoplazmoze i neke uvjetovane infekcijske bolesti životinja (Šeol, Branka, MZOS ) ( CroRIS)
053-0532400-2398 - Zdravstveni nadzor divljači (Slavica, Alen, MZOS ) ( CroRIS)
053-1430115-2116 - Molekularna epizootiologija i epidemiologija leptospiroze (Turk, Nenad, MZOS ) ( CroRIS)
068-1430115-2119 - Šumski ekosustavi kao prirodna žarišta hantavirusa i leptospira (Margaletić, Josip, MZOS ) ( CroRIS)