Pregled bibliografske jedinice broj: 318595
Structure and activity of an atrazine-degrading community enriched from a herbicide factory effluent
Structure and activity of an atrazine-degrading community enriched from a herbicide factory effluent // Power of microbes in industry and environment 2007 / Kosalec, Pigac, Vujaklija (ur.).
Zagreb: Pressum, 2007. (poster, nije recenziran, sažetak, znanstveni)
CROSBI ID: 318595 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Structure and activity of an atrazine-degrading community enriched from a herbicide factory effluent
Autori
Udikovic Kolic, Nikolina ; Hrsak, Dubravka ; Devers, Marion ; Martin-Laurent, Fabrice
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Power of microbes in industry and environment 2007
/ Kosalec, Pigac, Vujaklija - Zagreb : Pressum, 2007
ISBN
978-953-96567-5-9
Skup
Power of microbes in industry and environment
Mjesto i datum
Zadar, Hrvatska, 19.09.2007. - 22.09.2007
Vrsta sudjelovanja
Poster
Vrsta recenzije
Nije recenziran
Ključne riječi
atrazine; bacterial community; atz genes; trz genes
Sažetak
The objective of this work was to study diversity, genetic potential and catabolic activity of an atrazine-degrading bacterial community enriched from technological wastewater of a herbicide factory. This community mineralized 81.4 1.9% of [14C-ring] atrazine and 31.0 1.8% of [14C-ethyl] atrazine within 6-day batch cultivation in mineral salts medium containing atrazine as the only nitrogen source. 16S-RFLP analysis revealed high diversity of bacterial populations forming the community, with Pseudomonas species predominating. Atrazine-degrading genetic potential of the community determined by PCR, revealed the presence of trzN, atzB, atzC and trzD genes. The trzN, atzB, atzC genes were shown to be located on a single plasmid together with the insertion sequence(s) IS1071, while the location of trzD remains unknown. Further study of the density of functional community estimated by real-time PCR suggested that relative abundances of atz and trz genes were 10-fold to 22-fold lower than 16S rDNA indicating that atrazine-degrading populations represent only a fraction of the community. The obtained results also suggested that, in addition to plasmid conjugation, IS could play a role in dispersing atrazine-catabolic genes within the wastewater microbiota. Furthermore, besides the combined metabolic activities within the community, a different type of interactions may exist that enable sharing carbon and nitrogen from atrazine mineralization.
Izvorni jezik
Engleski
