Naslov
Combined metabolic activity within an atrazine-mineralizing community enriched from agrochemical factory soil

Autori
Udiković Kolić, Nikolina ; Hršak, Dubravka ; Begonja Kolar, Ana ; Petrić, Ines ; Stipičević, Sanja ; Soulas, Guy ; Martin-Laurent, Fabrice

Izvornik
International Biodeterioration and Biodegradation (0964-8305) 60 (2007), 4; 299-307

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
atrazine; biodegradation; atz; trz; microbial community

Sažetak
Aims: To characterize an atrazine-mineralizing community originating from agrochemical factory soil, especially to elucidate the catabolic pathway and individual metabolic and genetic potentials of culturable members. Methods and Results: A stable four-member bacterial community, characterized based on colony morphology and partial 16S rDNA sequencing, was capable to rapidly mineralize atrazine to CO2 and NH3. Two primary organisms were identified as Arthrobacter species (ATZ1 and ATZ2) and two secondary organisms as Ochrobactrum sp. (CA1) and Pseudomonas sp. (CA2). Atrazine-degrading genetic potential of the community estimated by PCR, revealed the presence of trzN, trzD, atzB and atzC genes. Isolates ATZ1 and ATZ2 were capable of dechlorinating atrazine to hydroxyatrazine and contained the trzN gene. ATZ2 continued to degrade hydroxyatrazine to cyanuric acid and contained atzB and atzC genes whereas ATZ1 contained atzC gene only. Isolates CA1 and CA2 grew on cyanuric acid and contained trzD gene. Conclusions: Complete atrazine degradation was a result of the combined metabolic attack on atrazine molecule, and complex interactions might exist between the community members sharing carbon and nitrogen from atrazine mineralization. Significance and Impact of the Study: This study will contribute towards better understanding of the genetic potential and metabolic activities of atrazine-degrading communities, which are generally considered to be more responsible for atrazine mineralization in natural environment than individual degrading species.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija

POVEZANOST RADA