Naslov
Genome organization and phylogeny of Anemone complex based on repetitive DNA

Autori
Mlinarec, Jelena ; Papeš, Dražena ; Besendorfer, Višnja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XIIth OPTIMA Meeting : Abstracts of the Lectures Comunication Posters / - Pisa, 2007, 220-220

Skup
OPTIMA Meeting (12 ; 2007)

Mjesto i datum
Pisa, Italija, 10.09.2007. - 16.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Anemone; chromosome evolution; repetitive DNAs

Sažetak
Anemone is one of the most interesting genera within Ranunculaceae family because of the variation in DNA content, high heterochromatin amount and its variation in distribution, and chromosome polymorphism. Previous results suggest that repetitive DNA abundance and distribution is involved in DNA content variation between members of the genus rather than changes in chromosome number. Our study was focused on characterization of repetitive DNAs and their implication on genome evolution in Anemone complex. Two satellite DNA (stDNA) families (JME and JMH2) and one dispersed family (JMH1), isolated from the Anemone hortensis genome, were analyzed in 11 species of the genus Anemone, 1 species of the genus Hepatica and 4 species of the genus Pulsatilla. JME is considered as intercalary and JMH2 as subtelomeric stDNA. Both stDNAs cover all the heterochromatic regions while the JMH1 repeat family revealed dispersed organization on all A. hortensis chromosomes. JMH1 exhibited partial homology to the Ty3/gypsy-like retroelement. PCR and Southern hybridization data revealed the widespread occurrence of JMH2 in all three investigated genera Anemone, Pulsatilla and Hepatica. JMH1 family was present in the genera Anemone and Hepatica but absent in the genus Pulsatilla. JME family is genus specific, i. e. present only in the 5 of 11 Anemone species examined. Phylogenetic analysis showed JMH1 sequence family to be informative for establishing relationships between species in section Anemone. The phylogenetic and evolutionary relationships among Anemone complex are discussed on the basis of repetitive DNA sequences and their position on chromosomes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA