Pregled bibliografske jedinice broj: 316692
Isolation of mussel metallothionein as biomarker of metal exposure
Isolation of mussel metallothionein as biomarker of metal exposure // Marine Chemistry - Analytical methods, trace elements and organic matter in marine environment / Plavšić, Marta (ur.).
Zagreb: Institut Ruđer Bošković, 2007. str. 26-27 (demonstracija, nije recenziran, sažetak, ostalo)
CROSBI ID: 316692 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Isolation of mussel metallothionein as biomarker of metal exposure
Autori
Raspor, Biserka ; Erk, Marijana
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo
Izvornik
Marine Chemistry - Analytical methods, trace elements and organic matter in marine environment
/ Plavšić, Marta - Zagreb : Institut Ruđer Bošković, 2007, 26-27
ISBN
978-953-6690-71-8
Skup
Marine Science and Coastal Management in the Adriatic, Western Balkans ; Course 3
Mjesto i datum
Zagreb, Hrvatska, 12.11.2007. - 17.11.2007
Vrsta sudjelovanja
Demonstracija
Vrsta recenzije
Nije recenziran
Ključne riječi
metallothionein; biochemical isolation
Sažetak
Lysis of cells is accomplished using a motorised glass/teflon homogeniser. During the homogenizing procedure the samples should be kept constantly cooled (on ice) and all buffers added should be cold. Beta-mercaptoethanol is added to protect protein thiols from oxidation. The supernatant obtained after centrifugation at 50000 x g for 120 min is called the S50-fraction and contains the cytosol.
Izvorni jezik
Engleski
Znanstvena područja
Kemija, Biologija
POVEZANOST RADA
Projekti:
098-0982934-2721 - Stanične promjene u vodnih organizama pobuđene metalima (Erk, Marijana, MZOS ) ( CroRIS)
Ustanove:
Institut "Ruđer Bošković", Zagreb
