Naslov
Trichomonas tenax proteolytic activity

Autori
Šegović, Sanja ; Buntak-Kobler Dunja ; Galić, Nada ; Katunarić, Marina

Izvornik
Collegium antropologicum (0350-6134) 22 (1998), Suppl. S; 45-49

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
trichomonas tenax proteolytic activity ; dentobacterial plaque ; polyacrylamide gels

Sažetak
In this study, proteolytic activity of Trichomonas tenax collected directly from patient's dentobacterial plaque was examined. Electrophoretic method involving polyacrylamide gels (Commassie Brilliant Blue R-250) and electrophoretic method involving gelatin- containing polyacrylamide gels, have been used to analyse Trichomonas tenax proteolytic activity. The most obvious and the fastest activities were obtained when gels were incubated in pH 4.6 ; followed by results of incubating in pH 5.6 ; while in pH 2.8 activity was less effective but still obvious. Proteolytic activities were the most effective in area of protein MW 36 kDa. Different activities of enzymes depending on pH of incubated media indicate the presence of different endopeptidase in cell lysates of protozoon Trichomonas tenax from dentobacterial plaque.

Izvorni jezik
Engleski

Znanstvena područja
Dentalna medicina

POVEZANOST RADA