Naslov
Comparison of thiobarbituric acid reactive substances (TBARS)and protein carbonyl (PC)as biomarkers of oxidative stress in rat (Ratus norvegicus)and common carp (Cyprinus carpio)
(Comparison of thiobarbituric acid reactive substances (TBARS) and protein carbonyl (PC) as biomarkers of oxidative stress in rat (Ratus norvegicus)and common carp (Cyprinus carpio))

Autori
Stepić, Sandra ; Velki, Mirna ; Hackenberger Kutuzović, Branimir ; Jarić-Perkušić, Davorka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the 5th Croatian Congress of Pharmacology and the 2nd Congress of Croatian Physiological Society ; u: Periodicum biologorum. Supplement / - Osijek, 2007, 158-158

Skup
Croatian Congress of Pharmacology (5 ; 2007) ; Congress of Croatian Physiological Society (2 ; 2007)

Mjesto i datum
Osijek, Hrvatska, 19.09.2007. - 22.09.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
oxidative stress; TBARS; protein carbonyl; rat; carp

Sažetak
Oxidative stress can be defined as an imbalance between oxidative-reductive processes due to overproduction of free oxygen radicals that cell homeostatic mechanisms can not neutralize, which leads to harmful biochemical and physiological alterations in cells. Two biochemical alterations resulting from oxidative stress are lipid peroxidation and protein carbonyl formation. There are many conditions that can cause increased free radical production and induce oxidative stress, for example toxic substance exposure, smoking, alcohol consumption, as well as exercise at high intensity. The aim of this work was to evaluate the effects of intensive exercise on concentration of thiobarbituric acid reactive substances (TBARS) and protein carbonyl (PC) concentration as biomarkers of oxidative stress, in rat (Ratus norvegicus) and common carp (Cyprinus carpio). For that purpose, a total of 20 male rats were randomly divided into two groups. Group A was the control group (n=10) and did not perform exercise and group B was the experimental group (n=10) that was exposed to intensive exercise on treadmill over a two week period (120 min/day at 1.6 km/h for 5 days/wk). One group of rats was sacrificed after first week (5 rats from both groups) and another group (residual rats) was sacrificed at the end of the experiment, liver was collected from each animal for estimation of TBARS and PC concentrations. Fish were also divided into two groups. Group A was the control group (n=14) and did not perform exercise and group B was the experimental group (n=14) that was exposed to intensive exercise (8 h/day over a 6 days) in 150L pool in which a system for water mixing was constructed (water flow was 15 cm/s). One group of fish was sacrificed after three days (7 fish from both groups) and another group (residual fish) was sacrificed at the end of the experiment, liver was collected from each carp for estimation of TBARS and PC concentrations. The results showed that the acute intensive exercise induced significant increase in rat liver TBARS concentrations after one week in the B group, yet after two week period liver TBARS concentrations were lower in this group of rats. The PC levels after first week of experiment were not significantly increased in the B group of rats, but after two week period a significant increase in the PC levels was observed. The resembling results were obtained with the carp experiment. Significant increase of TBARS levels in carp were obtained after three days of exposure to intensive exercise, although these levels already started to decrease after six days. Significant increase in PC level in carp that were exposed to intensive exercise were obtained after six days of exposure. The levels of TBARS after oxidative stress caused by increased free radical production after intensive exercise increased much earlier than the PC concentrations levels, although the PC levels maintained increased much longer than the TBARS levels. That should be taken into account when using these two biomarkers in the environmental biomonitoring.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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