Naslov
Emergence of Uropathogenic Extended-Spectrum Beta Lactamases-Producing Escherichia coli Strains in the Community

Autori
Marijan, Tatjana ; Vraneš, Jasmina ; Bedenić, Branka ; Mlinarić-Džepina, Ana ; Plečko, Vanda ; Kalenić, Smilja

Izvornik
Collegium Anropologicum (0350-6134) 31 (2007), 1; 227-233

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
urinary tract infections; Escherichia coli; virulence; extended-spectrum beta-lactamases; clonal dissemination

Sažetak
The aim of this study was to determine the virulence characteristics and resistance pattern of the extended-spectrum beta lactamases (ESBLs)-producing Escherichia coli strains isolated from urine of outpatients in the Zagreb region during a five-month period, and to compare them with the non ESBLs-producing E. coli strains isolated in the same period. Out of 2, 451 E. coli strains isolated from urine of nonhospitalized patients with significant bacteriuria, a total of 39 ESBLsproducing strains (1.59%) were detected by a double-disk diffusion technique and by the broth-dilution minimal inhibitory concentration reduction method. The 45 non ESBLs-producing strains were randomly chosen, and phenotype of the two groups of strains was characterized and compared. Serogroup O4, hemolysin production, expression of P- and type 1 fimbriae as well as resistance to gentamicin and amikacin were significantly more prevalent characteristics among the ESBLs-producing strains than among non ESBLs-producing strains (p<0.01), while higher prevalence of trimethoprim-sulfamethoxazole resistance among ESBLs-producing strains was not statistically significant (p>0.05). Chromosomal DNA analysis by pulsed-field gel electrophoresis exhibited a great genomic similarity among ESBLs-producing strains and revealed that those highly virulent and resistant E. coli strains isolated from urine of outpatients in the Zagreb region had a clonal propagation.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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