Naslov
Microphthalmia-associated transcription factor and tyrosinase as markers of melanoma cells in blood of patients with melanoma

Autori
Šamija, Ivan ; Lukač, Josip ; Marić-Brozić, Jasmina ; Šitum, Mirna ; Končar Mubrin, Mirjana ; Kusić, Zvonko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Annual Meeting of the Croatian Immunological Society 2005

Mjesto i datum
Božava - Dugi otok, Hrvatska, 29.09.2005. - 02.10.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
mikroftalmijski transkripcijski faktor; tirozinaza; melanom; cirkulirajuće tumorske stanice
(microphthalmia-associated transcription factor; tyrosinase; melanoma; circulating tumor cells)

Sažetak
Reverse trasnscription – polymerase chain reaction (RT-PCR) for tyrosinase mRNA is specific and sensitive method for detection of circulating melanoma cells in peripheral blood of melanoma patients. The major limitation in clinical use of tyrosinase as a prognostic marker is a relatively high number of melanoma patients with distant metastases being tyrosinase negative. Therefore, additional markers to tyrosinase for RT-PCR detection of circulating melanoma cells are being investigated. Microphthalmia-associated transcription factor (MITF) has shown high sensitivity and specificity as a marker for immunohistochemical diagnosis of melanoma. The aim of this study was to investigate whether analysis of MITF as an additional marker to tyrosinase in melanoma patients can improve the detection of circulating melanoma cells by RT-PCR. Blood samples were taken from 201 patients with melanoma in all stages of the disease. RNA was isolated from mononuclear cell fraction of the blood and reversely transcribed into the cDNA. The cDNA was assayed by PCR for the expression of tyrosinase and MITF. Blood samples from 40 healthy subjects were used as controls. The threshold for detection of both tyrosinase and MITF was set low enough to detect 50 melanoma cells in 10 mL of healthy volunteer blood. Out of 201 blood samples from melanoma patients, 16 were positive for tyrosinase only, 28 for MITF only, and 4 for both tyrosinase and MITF. All samples from healthy volunteers were negative for both tyrosinase and MITF. Determination of MITF marker in addition to tyrosinase improved the detection of circulating melanoma cells in patients with melanoma.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti

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