Naslov
Analysis of clonality in T-lymphoprolifertive diseases by multiplex PCR

Autori
Zadro, Renata ; Kralj, Danijela ; Aurer, Igor ; Kovačević-Metelko, Jasminka ; Labar, Boris ; Stavljenić-Rukavina, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of The Second Croatian Congress in Human Genetics ; u: Paediatria Croatica 42 (1998) (S3) / Stavljenić-Rukavina, Ana - Zagreb : Klinika za pedijatriju, 1998, 40-40

Skup
Croatian Congress in Human Genetics (2 ; 1998)

Mjesto i datum
Zagreb, Hrvatska, 21.10.1998. - 24.10.1998

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
PCR; TCR-gamma; acute leukemia; diagnosis

Sažetak
Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix the patient specific T-cell receptor gamma-rearrangement was determined: five of ten patients showed the exclusive presence of a single T-cell receptor gamma-gene rearrangement. Three patients exhibited two rearranged TCR-gamma genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segment. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represnets a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA