Naslov
Imp and secY, two new markers for MLST (multilocus sequence typing) in the 16SrX phytoplasma taxonomic group

Autori
Danet, Jean-Luc ; Bonnet, Patrick ; Jarausch, Wolfgang ; Carraro, Luigi ; Škorić, Dijana ; Labonne, Gerard, Foissac, Xavier

Izvornik
Bulletin of Insectology (1721-8861) 60 (2007), 2; 339-340

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Mollicutes; variability; fruit tree diseases; pear decline; apple proliferation; European stone fruit yellows; aceF; pnp; phylogenetic analyses

Sažetak
To investigate the genetic variability of fruit tree phytoplasmas belonging to the 16SrX phylogenetic group, we used a Multilocus Sequence Typing strategy (MLST). Sequences of four non-ribosomal genetic loci (aceF, pnp, imp and secY) were determined among a collection of Candidatus Phytoplasma prunorum, Ca. P. mali and Ca. P. pyri isolates. Sequences alignment and phy-logenetic analyses confirmed the classification based on rDNA16S phylogeny. The four genetic loci displayed specific signatures clearly correlated with the recent definition of the corresponding Candidatus species. However, a total congruence was not ob-served inside species, between phylogenetic trees constructed with the different loci. A divergent Ca. P. prunorum variant from Azerbaijan, recently characterized through aceF sequencing, again presented a divergent imp gene. The previously characterized Ca. P. prunorum hypovirulent strains had the same aceF sequence but were discriminated with the imp marker. Ca.P. pyri strains detected in Lebanese Pear Decline isolates also form a distinct cluster according to aceF and imp markers. Imp and secY phyloge-netic analyses pointed out a new Ca. P. prunorum cluster comprising strains never detected in psyllid vectors (non circulative strains ?). On the opposite, the circulative Ca.P. prunorum strains were distributed among all phylogenetic group identified. For Ca. P.mali, discrimination of apple proliferation isolates was in agreement with previous studies.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija)

Napomena
Volumen 60(2) časopisa Bulletin of Insectology sadržava radove First International Phytoplasmologist Working Group Meeting održanog u Bologni (12-15. 11. 2007.).

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