Naslov
Dendritic CD1a+ cells regulate the expansion of CD3+ cells at the maternal fetal interface

Autori
Rubeša, Željka ; Laškarin, Gordana ; Redžović, Arnela ; Veljković, Danijela ; Haller, Herman ; Allavena, Paola ; Mantovani, Albrto ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / Rabatić, Sabina - Zagreb : Hrvatsko imunološko društvo, 2007, 28-28

Skup
2007 Annual Meeting of the Croatian Immunological Society

Mjesto i datum
Rovinj, Hrvatska, 19.10.2007. - 21.10.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
decidual mononuclear cells; cytotoxicity; pregnancy; cytolytic mediators

Sažetak
The study was aimed to investigate cytolytic potential of CD1a+ cells towards CD3+ cells of the first trimester pregnancy decidua. Decidual mononuclear cells (DMC) were obtained by enzimatic digestion and gradient density centrifugation. The expression of NK receptors has been analyzed in CD1a+CD56+ and CD1a+CD56- cell subpopulations. Cytolytic mediators (perforin, granulysin, FasL, TRAIL and TNF-α ) were analysed in CD1a+ cells using cell permeabilisation method. The method for the isolation and purification of decidual CD1a+ and CD3+ cells was established. Decidual CD1a+ cells were positively selected from the adherent DMC fraction, while decidual CD3+ cells were obtained from CD56-CD83- DMC fraction using nanobeads conjugated α -CD6 antibody. Purified CD1a+ cells, as the effectors, were set up with target CD3+ cells at different cell-ratios. The mechanism of CD1a+ cells cytotoxicity towards CD3+ cells was tested using 18 hrs PKH-26 (red) assay and α -perforin, α -FasL, α -TRAIL and α -granulysin antibodies. The results have shown that both CD1a+CD56+ and CD1a+CD56- subpopulations express NK receptors NKG2A, NKG2C, NKG2D, CD94, NKp30 and NKp46. The expression of cytolytic mediators (perforin, FasL, TRAIL and granulysin) enable them to kill autologuous CD3+ cells prefentially using perforin and FasL mechanism. This data point out decidual CD1a+ cells as the important factor for the regulation of T cells frequency in early pregnancy decidua. Acnowledgement: The experiments are financed by Croatian Ministry of Science, grant No. 062-0620402-0376 and EMBIC project, European FP6 project No. 512040, NoE, LSHM-CT-2004-512040

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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