Naslov
Interactions between HLA-G molecule and decidual natural killer cells of human first trimester pregnancy

Autori
Juretić Franković, Koraljka ; Laškarin, Gordana ; Haller, Herman ; Vlastelić, Ivan ; Le Bouteiller, Philippe ; Rukavina, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
American Journal of Reproductive Immunology / Beaman, Kenneth ; Rukavina, Daniel - Oxford : Wiley-Blackwell, 2007, 431-431

Skup
X International Congress of Reproductive Immunology

Mjesto i datum
Opatija, Hrvatska, 10.06.2007. - 14.06.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
HLA-G molecule; decidual natural killer cells; human first trimester pregnancy; cytotoxicity; decidua

Sažetak
Problem: Non-classical MHC-I molecule HLA-G, selectively expressed on invading extravillous cytotrophoblast, is in close contact with decidual CD56+ NK cells infiltrating first trimester decidua. We showed recently that exposure to HLA-G positive trophoblast altered their phenotype and function (decidual NK cells were able to induce FasL protein expression in close contact with HLA-G molecule and it represented dominant mechanism of cytotoxicity for K562-HLA-G cells). Therefore, our aim was to investigate further influence of this molecule on decidual NK cells. Methods of study: Decidual tissue was minced, treated with collagenase and passed through density gradient. After centrifugation, decidual mononuclear cells were collected. Intracellular expression of cytolytic mediators, NK cell activating receptors and Th1 cytokines was determined in decidual CD56+ cells after their 18 hour-culture with K-562-PWD cells and K-562-HLA-G transfectants at 37 C (1:1). Immunohistology for FasL and TRAIL was performed. Results: After overnight culture, decidual NK cells decreased their content of perforin in close contact with HLA-G molecule, comparing to their co-culture with K-562-PWD. We also observed HLA-G mediated decrease of activating NK receptor expression (NKG2D, NKG2C, NKp44, NKp46) as well as Th1 cytokine expression (IFN- , IL-18, TNF- ) in decidual NK cells. FasL and TRAIL were detected on paraffin-embedded first trimester decidual tissue sections as well as FasL by using Alexa 555. Conclusions: HLA-G decreased expression of cytolytic mediators, NK cell activating receptors and Th1 cytokines in decidual CD56+ cells. Since expression of HLA-G molecule is limited to gestation, it seems to be important in the regulation of various decidual NK cell functions. The experiments are partially financed by Croatian Ministry of Science (Grants No. 0620402-0376 and 0379) and EMBIC project, European FP6, NoE, LSHM-CT-2004-512040.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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