Naslov
Evaluation of genotoxic potential of radiofrequency electromagnetic field (RF-EMF) in duckweed

Autori
Cvjetko, Petra ; Radić, Sandra ; Malarić, Krešimir ; Tkalec, Mirta ; Pavlica, Mirjana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Environmental and Molecular Mutagenesis Vol. 48. Special Issue. Abstracts / - , 2007, 614-614

Skup
Environmental Mutagen Society 38th Annual Meeting

Mjesto i datum
Atlanta (GA), Sjedinjene Američke Države; Gruzija, 20.10.2007. - 24.10.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
radiofrequency electromagnetic field; duckweed; genotoxicity; comet assay; DNA

Sažetak
The use of wireless communication devices has drawn considerable scientific and public attention to the issue of whether or not adverse effects result from exposure to radiofrequency radiation, especially those emitted by handheld mobiles. In the mean time, rapidly emerging modern technologies in the world of telecommunications, have led to the electromagnetic pollution which became important as much as the industrial pollution of different sources. Because of reasonable concern that electromagnetic radiation may constitute a risk to human health, many in vitro and in vivo studies have been performed in order to define the biological mechanism for the better understanding of how the above mentioned radiation exerts its effect. So far this still remains unknown. Among many different methods and techniques, the single cell gel electrophoresis, also known as the comet assay is one of the most commonly used toxicity tests to detect the unknown effect of many chemical and physical agents. The same method has also found its applicability in search for possible adverse effect of electromagnetic radiation on the integrity of DNA molecule. So far the genotoxicity evaluation of electromagnetic radiation by using the comet assay was mainly performed on animal and mammalian cells. The aim of this study has been to investigate the possibility of using the alkaline protocol of the comet assay as a method for detecting DNA damage caused by radiofrequency electromagnetic fields (RF-EMF) in plant cells. Higher plants offer unique advantages as test systems such as easiness of handling, low cost, vegetative reproduction and most important good correlation to animal test systems. Duckweed plants were exposed to continuous waves EMF at field strength of 23 V/m for 4 h in the Gigahertz Transversal Electromagnetic (GTEM) cell, which according to the literature exclude thermal stress. Evaluation of DNA damage in duckweed cultures exposed to 900 MHz for 4 hour has shown significant increase in DNA stand breaks compared to control sample. This findings clearly indicate that plants are suitable model system for evaluation of genotoxic potential of RF-EMF.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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