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Pregled bibliografske jedinice broj: 309342

Proteomic characterization of inter-alpha inhibitor proteins from human plasma


Josić, Djuro; Brown, Mari Kino; Huang, Feilei; Lim, Yow-Pin; Ručević, Marijana; Clifton, James G.; Hixson, Douglas C.
Proteomic characterization of inter-alpha inhibitor proteins from human plasma // Proteomics, 6 (2006), 9; 2874-2885 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 309342 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Proteomic characterization of inter-alpha inhibitor proteins from human plasma

Autori
Josić, Djuro ; Brown, Mari Kino ; Huang, Feilei ; Lim, Yow-Pin ; Ručević, Marijana ; Clifton, James G. ; Hixson, Douglas C.

Izvornik
Proteomics (1615-9853) 6 (2006), 9; 2874-2885

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
biomarkers; diagnostic and therapeutic agents; inter-alpha inhibitor proteins

Sažetak
Inter-alpha inhibitor proteins (IaIp) are a family of structurally related serine protease inhibitors found in relatively high concentrations in human plasma. Recent studies have implicated a role for IaIp in sepsis, and have demonstrated their potential as biomarkers in sepsis and cancer. For characterization of isolated IaI proteins and contaminating proteins during the last steps of the purification process, SELDI-TOF MS and HPLC– ESI-MS/MS were used. After separation by SDSPAGE or 2-DE, polypeptide bands of 80, 125 and 250 kDa were excised from gels and digested by trypsin. The tryptic peptides were analyzed by bothMS methods. The main contamination during the purification process, a band of 80 kDa, contains mainly IaIp heavy chain (HC) H3.HCH1 and H2 were also found in this band. In addition, some vitamin K-dependent clotting factors and inhibitors and other plasma proteins were identified. The 125-kDa band, representing the pre-alpha inhibitor, was found to contain both bikunin and HC H3. The presence of other HC H1, H2 and the recently described HC H4 was also detected by SELDI-TOF MS. The presence of HC H1, H2, and H3 in the 125-kDa band was confirmed by ESI-MS/MS, but not the presence of the H4. Three polypeptides, H1 and H2 together with bikunin, were identified in the 250-kDa band, representing the ITI, by bothMS techniques. Once again, the presence ofH4 was detected in this band only by SELDI-TOF MS, but the number of corresponding peptides was still not sufficient for final identification of this polypeptide. The importance of the application of proteomic methods for the proper evaluation of therapeutic drugs based on human plasma is discussed.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Biotehnologija

Napomena
Rad ostvaren u suradnji s: Proteomics Core, COBRE Center for Cancer Research Development, Rhode Island Hospital, Providence, RI, USA i Division of Medical Oncology, Department of Medicine, Rhode Island Hospital, Providence, RI, USA



POVEZANOST RADA


Ustanove:
Prehrambeno-biotehnološki fakultet, Zagreb

Profili:

Avatar Url Marijana Ručević (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada

Citiraj ovu publikaciju:

Josić, Djuro; Brown, Mari Kino; Huang, Feilei; Lim, Yow-Pin; Ručević, Marijana; Clifton, James G.; Hixson, Douglas C.
Proteomic characterization of inter-alpha inhibitor proteins from human plasma // Proteomics, 6 (2006), 9; 2874-2885 (međunarodna recenzija, članak, znanstveni)
Josić, D., Brown, M., Huang, F., Lim, Y., Ručević, M., Clifton, J. & Hixson, D. (2006) Proteomic characterization of inter-alpha inhibitor proteins from human plasma. Proteomics, 6 (9), 2874-2885.
@article{article, author = {Josi\'{c}, Djuro and Brown, Mari Kino and Huang, Feilei and Lim, Yow-Pin and Ru\v{c}evi\'{c}, Marijana and Clifton, James G. and Hixson, Douglas C.}, year = {2006}, pages = {2874-2885}, keywords = {biomarkers, diagnostic and therapeutic agents, inter-alpha inhibitor proteins}, journal = {Proteomics}, volume = {6}, number = {9}, issn = {1615-9853}, title = {Proteomic characterization of inter-alpha inhibitor proteins from human plasma}, keyword = {biomarkers, diagnostic and therapeutic agents, inter-alpha inhibitor proteins} }
@article{article, author = {Josi\'{c}, Djuro and Brown, Mari Kino and Huang, Feilei and Lim, Yow-Pin and Ru\v{c}evi\'{c}, Marijana and Clifton, James G. and Hixson, Douglas C.}, year = {2006}, pages = {2874-2885}, keywords = {biomarkers, diagnostic and therapeutic agents, inter-alpha inhibitor proteins}, journal = {Proteomics}, volume = {6}, number = {9}, issn = {1615-9853}, title = {Proteomic characterization of inter-alpha inhibitor proteins from human plasma}, keyword = {biomarkers, diagnostic and therapeutic agents, inter-alpha inhibitor proteins} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE





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