Naslov
Modelling of L-DOPA oxidation catalyzed by laccase

Autori
Tišma, Marina ; Žnidaršič-Plazl, Polona ; Plazl, Igor ; Zelić, Bruno ; Vasić-Rački, Đurđa

Izvornik
Chemical and Biochemical Engineering Quarterly (0352-9568) 22 (2008), 3; 307-313

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
L-DOPA; laccase; enzymatic oxidation; modelling

Sažetak
The enzymatic oxidation of 3, 4-dihydroxyphenyl-L-alanine (L-DOPA) with laccase from Trametes versicolor has been investigated. The highest enzyme activity at pH 5.4 and at 25 º C was found. The reaction kinetics and the effect of dissolved oxygen concentration on the reaction rate were evaluated. A mathematical model, comprised of double-substrate Michealis-Menten kinetics and mass balances for L-DOPA and dissolved oxygen concentrations was developed in order to describe and to predict the process of L-DOPA oxidation. Kinetic parameters, Vm, Km(L-DOPA) and Km(O2) have been estimated and experimentally verified by a set of experiments with constant additional aeration for different initial concentrations of L-DOPA and dissolved oxygen. A significant increase in reaction rate at higher oxygen concentration in the inlet gas has been established. Using the developed model the influence of dissolved oxygen concentration on L-DOPA conversion was investigated.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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