Naslov
Quantitative Protein Biomarker Analysis using Monolithic ImmunoDisks

Autori
Y.P. Lim, M. Ručević.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Summer School on Monolith Technology for Biochromatography, Bioconversion, and Solid Phase Synthesis

Mjesto i datum
Portorož, Slovenija, 28.05.2006. - 31.05.2006

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Inter-alpha inhibitor; Granzym K; Protein biomarker; Sepsis; Monolithic ImmunoDisks

Sažetak
Immunoassays are analytical methods based on signal responses generated as a consequence of an antibody-antigen reaction. Since they were first reported, immunoassays have witnessed phenomenal growth in the range and scope of their applications. A vast array of different labels and assay strategies has been developed to meet requirements of sensitivity, accuracy and convenience in analysis of biomolecules. They are widely used in such important areas as diagnostic biomarkers for various disease states and for assessment of progression or regression during therapy intervention as well as in drug development to provide important information about the safety and efficacy of candidate drugs. While conventional immunoassay such as enzyme-linked immunosorbant assays (ELISA) needs multiple reagents or labels, we attempt to develop a simple and rapid chromatographic based assay using monolithic CIM disks. We are currently investigating the roles of Inter-alpha inhibitor (IaI) and its physiological substrate, Granzyme K (GrK) in the pathogenesis of systemic inflammation/sepsis. The levels of these molecules in plasma of patients with sepsis were significantly altered compared to healthy individuals. Moreover, the plasma levels of IaI and GrK seem to correlate with the severity of the disease, suggesting the potential clinical utilization as useful predictive markers. Specific antibody reagents generated against IaI and GrK are immobilized on Epoxy-activated CIM minidisks to capture these molecules directly from plasma. Standard curves are developed by using known amounts of affinity purified IaI and GrK. Analysis of both biomarkers is performed separately or in tandem for simultaneous measurement of multiple circulating plasma biomarkers as a model for multiplex immunoassay. CIM ImmunoDisks provide a rapid and simple analysis of biomarkers in clinical arena as well as in clinical drug development.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Biotehnologija

POVEZANOST RADA