Naslov
HPV 16 DNA integration in precancerous cervical lesions.

Autori
Matovina, Mihaela ; Sabol, Ivan ; Milutin Gašperov, Nina ; Grce, Magdalena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Proceedings of the Abstracts of the The 12th World Congress on Advances in Oncology, and 10th International Symposium on Molecular Medicine / Spandidos, D.A. - Atena : LYCHNIA, 2007, S80-S80

Skup
The 12th World Congress on Advances in Oncology, and 10th International Symposium on Molecular Medicine

Mjesto i datum
Hersonissos, Grčka, 11.10.2007. - 13.10.2007

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Human papillomaviruses (HPV); integration

Sažetak
The integration of HPV DNA in the host cell genome is the key event in the development of cervical cancer. The site of integration within the genome is random, although preferential integration in common fragile sites (CFS) have been proposed. It is not clear if the site of integration in the host cell genome could have an impact on the development of cervical cancer. Thus, a study was conducted on cervical precancerous lesions (LSIL and HSIL) in order to determine the frequency and the sites of viral integration in the host cell genome in HPV 16 positive cervical samples by DIPS-PCR (Detection of Integrated Papillomavirus Sequences Polymerase Chain Reaction) method according to Luft et al. (Int J Cancer 2001 ; 92: 9-17) with our modifications. Thirty of 176 samples were not suitable for the analysis by DIPS-PCR. In 92.5% (135/146) samples, episomal form of HPV 16 DNA was determined, while integrated alone (8 cases) or mixed (3 cases) forms of HPV 16 DNA were found in 7.5% (11/46) samples. In 23 LSIL samples we did not find integrated form of HPV 16 DNA, while in the HSIL samples, we found integrated form in 12.3% (8 integrated and 2 mixed out of 81) samples. Also, in one sample of 42 (2.4%) with unknown cytological diagnosis mixed form of HPV16 DNA was found. The site of integration in all 11 cases was random. However, in 63.6% (7/11) cases HPV16 DNA was integrated within the CFS in the genome and in 54.5% (6/11) cases HPV 16 DNA was integrated within the cellular genes, namely VMP1, CHERP, CEACAM5, PVRL1, ARID5B and AHR. Our findings indicate that HPV 16 DNA integration into the host cell genome is indeed a reliable marker of cervical neoplastic progression. Additionally, our findings confirm that HPV 16 DNA preferentially integrates within CFS in the genome, but also within cellular genes, which each of them might have a crucial role in the development of cervical cancer.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

Napomena
International Journal of Molecular Medicine 20 (2007) Suppl. 1

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