Naslov
Effects of speciffic immunotherapy on the CD45RA molecule in peripheral blood lymphocytes

Autori
Stipić-Marković, Asja ; Miculinić, Neven ; Klobučar, Hrvoje ; Čvorišćec, Branimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book / - Philadelphia (PA) : American College of Allergy, Asthma & Immunology, 1998

Skup
1998 annual meeting American College of Allergy, Asthma & Immunology

Mjesto i datum
Philadelphia (PA), Sjedinjene Američke Države, 06.11.1998. - 11.11.1998

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
immunotherapy; allergy; CD45RA; lymphocytes; Dermatophagoides pteronyssinus

Sažetak
The purpose of this study was to explore the influence of allergen-specific immunotherapy (ASIT) on CD45RA molecule expression in peripheral blood lymphocytes after allergen exposure. In a group of 31 patients (21 M, 10 F, age 15-37) sensitized to Dermatophagoides pteronyssinus, with clinical symptoms of asthma and/or perennial rhinitis, bronchial allergen challenge by dosimeter method was done, prior to and six months after ASIT, applied by the rush method. Reaction was defined as the dose that induced a 20% fall in FEV1. The allergen was Croatian National Standard with potency of 65300 BU/ampoute. Phenotypic analysis of lymphocytes was perfonned by flow cytometry (mAb Becton Dickinson), prior to and 48 hours after allergen-induced acute asthmatic attack. In parallel, absolute numbers of peripheral blood eosinophils were determined. The results showed that allergen stimulation induced increased expression of the CD45RA molecule on peripheral blood lymphocytes (p=0,008) together with increased eosinophil counts (p=0,0002), measured after 48 hours. All patients had an early bronchial reaction with a mean fall in FEV1 of 28ą8%. After six months of ASIT, expression of the CD45RA molecule, post allergen challenge, was inhibited in treated patients in comparison to the control group. Also, the increase in eosinophil counts was lower compared to pretreatment and control group values. We conclude that allergen stimulation induces significant increases in the expression of CD45RA molecules that is blocked by specific immunotherapy. This supports the idea that immunotherapy changes lymphocyte response to a specific allergen.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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