Naslov
Characterization of Secreted and Granular Granzyme K Purified from Human NK Cell Line

Autori
M. Ručević, D. Josić, Y.P. Lim, L. Fast.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
FASEB Journal 19(5) / - Bethesda (MD), 2005

Skup
FASEB Meeting Experimental Biology 2005 XXXV International Congress of Physiological Sciences

Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 02.04.2005. - 06.04.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
NK cell line; Granzym K; Granular; Secreted

Sažetak
The human granzymes (Grs) are a highly conserved group of specific proteases that are found in specialized granules of cytotoxic T lymphocytes and natural killer (NK) cells. Although several mechanisms of cell death induced by Grs have been elucidated and two distinct pathways of biosynthesis have been identified, the substrates and functions of many of the Grs have yet to be discovered. Studies characterizing human GrK were initiated based on our observations that the level of GrK correlated with the stage of sepsis. Previously characterized anti-GrK polyclonal antibody and commercially available monoclonal antibody that recognize human recombinant GrK (rGrK) were used for immunocharacterization of GrK purified from cytotoxic granules and cultured media of human NK cell line. GrK purified from cytotoxic granules was identified as specific band in SDS-PAGE corresponding in apparent molecular size to rGrK while secreted GrK appears in slightly larger molecular form. Both granule-related and secreted GrK cleaved synthetic substrate Z-Arg-Sbzl. In conclusion, we provide evidence that the GrK that is constitutively secreted differs from the form that is stored into cytotoxic granules of human NK cells, suggesting different pathways of biosynthesis and may indicate different biological functions. The role of these different forms of GrK in systemic inflammation is being investigated.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija

POVEZANOST RADA