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Pregled bibliografske jedinice broj: 307159

Coenzyme regeneration catalyzed by NADH oxidase from Lactobacillus brevis in the reaction of L-amino acid oxidation

Findrik, Zvjezdana; Šimunović, Ines; Vasić-Rački, Đurđa
Coenzyme regeneration catalyzed by NADH oxidase from Lactobacillus brevis in the reaction of L-amino acid oxidation // Biochemical engineering journal, 39 (2008), 2; 319-327 doi:10.1016/j.bej.2007.10.003 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 307159 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Coenzyme regeneration catalyzed by NADH oxidase from Lactobacillus brevis in the reaction of L-amino acid oxidation

Autori
Findrik, Zvjezdana ; Šimunović, Ines ; Vasić-Rački, Đurđa

Izvornik
Biochemical engineering journal (1369-703X) 39 (2008), 2; 319-327

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
amino acid ; coenzyme regeneration ; NADH oxidase ; L-phenylalanine dehydrogenase ; enzymes ; enzyme biocatalysis

Sažetak
In this paper L-methionine oxidation catalyzed by L-phenylalanine dehydrogenase from Rhodococcus sp. M4 was studied. It was found that the reaction equilibrium is shifted to the side of reduction, and it was therefore necessary to regenerate NAD+ to increase L-methionine conversion. NADH oxidase from Lactobacillus brevis was used for that purpose. The enzyme was kinetically characterized. It was found that the enzyme is inhibited by NAD+. Hence, NADH oxidation catalyzed by NADH oxidase was described by the Michaelis-Menten equation which included anticompetitive NAD+ inhibition. L- methionine oxidation was described by formal double-substrate Michaelis-Menten model which included competitive product inhibition by NADH. 2-oxo-4-methylthiobutyric acid reduction was described by formal three-substrate Michaelis- Menten kinetics which included competitive inhibition by NAD+. Experiments were carried out in the batch and in the continuously operated enzyme membrane reactor. 100 % L-methionine conversion was achieved in the batch reactor. The conversion was lower in the continuously operated enzyme membrane reactor where enzyme deactivation occurred.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija



POVEZANOST RADA

Projekti:
MZOS-125-1252086-2793 - Biokatalizatori i biotransformacije (Vasić-Rački, Đurđa, MZOS ) ( CroRIS)

Ustanove:
Fakultet kemijskog inženjerstva i tehnologije, Zagreb

Profili:

Avatar Url Đurđa Vasić-Rački (autor)

Avatar Url Zvjezdana Findrik Blažević (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi www.sciencedirect.com dx.doi.org

Citiraj ovu publikaciju:

Findrik, Zvjezdana; Šimunović, Ines; Vasić-Rački, Đurđa
Coenzyme regeneration catalyzed by NADH oxidase from Lactobacillus brevis in the reaction of L-amino acid oxidation // Biochemical engineering journal, 39 (2008), 2; 319-327 doi:10.1016/j.bej.2007.10.003 (međunarodna recenzija, članak, znanstveni)
Findrik, Z., Šimunović, I. & Vasić-Rački, Đ. (2008) Coenzyme regeneration catalyzed by NADH oxidase from Lactobacillus brevis in the reaction of L-amino acid oxidation. Biochemical engineering journal, 39 (2), 319-327 doi:10.1016/j.bej.2007.10.003.
@article{article, author = {Findrik, Zvjezdana and \v{S}imunovi\'{c}, Ines and Vasi\'{c}-Ra\v{c}ki, \DJur\dja}, year = {2008}, pages = {319-327}, DOI = {10.1016/j.bej.2007.10.003}, keywords = {amino acid, coenzyme regeneration, NADH oxidase, L-phenylalanine dehydrogenase, enzymes, enzyme biocatalysis}, journal = {Biochemical engineering journal}, doi = {10.1016/j.bej.2007.10.003}, volume = {39}, number = {2}, issn = {1369-703X}, title = {Coenzyme regeneration catalyzed by NADH oxidase from Lactobacillus brevis in the reaction of L-amino acid oxidation}, keyword = {amino acid, coenzyme regeneration, NADH oxidase, L-phenylalanine dehydrogenase, enzymes, enzyme biocatalysis} }
@article{article, author = {Findrik, Zvjezdana and \v{S}imunovi\'{c}, Ines and Vasi\'{c}-Ra\v{c}ki, \DJur\dja}, year = {2008}, pages = {319-327}, DOI = {10.1016/j.bej.2007.10.003}, keywords = {amino acid, coenzyme regeneration, NADH oxidase, L-phenylalanine dehydrogenase, enzymes, enzyme biocatalysis}, journal = {Biochemical engineering journal}, doi = {10.1016/j.bej.2007.10.003}, volume = {39}, number = {2}, issn = {1369-703X}, title = {Coenzyme regeneration catalyzed by NADH oxidase from Lactobacillus brevis in the reaction of L-amino acid oxidation}, keyword = {amino acid, coenzyme regeneration, NADH oxidase, L-phenylalanine dehydrogenase, enzymes, enzyme biocatalysis} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus

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  • CA Search (Chemical Abstracts)

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