Naslov
Protective effect of anti-LPS monoclonal antibody in intranasal model of Klebsiella pneumoniae infection

Autori
Ivica Franciškovic, Luka Bastiančić, Vanja Marchesi, Tomislav Rukavina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
CROSS 3 / - , 2007, 24-24

Skup
3rd International Croatian Student Summit

Mjesto i datum
Zagreb, Hrvatska, 29.03.2007. - 01.04.2007

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Klebsiella pneumoniae; protulipopolisaharidna imunost; intranazalna infekcija
(Klebsiella pneumoniae; anti-LPS immunity; intranasal infection)

Sažetak
The purpose of this work was to examine the effect of anti-LPS monoclonal antibody protection in mice intranasally challenged with a lethal dose of Klebsiella pneumoniae. In our experiments we used BALB/CN mice eight to twelve weeks old. They were separated in two groups each containing 6 animals. One group received 1 mg per animal of anti-LPS monoclonal antibodies and other received saline intraperitoneally 4 hours prior to the infection. We used anti-LPS monoclonal antibodies that were previously shown to be highly specific for O1 LPS of Klebsiella pneumoniae. Animals were infected with a lethal dose of K. pneumoniae strain Caroli O1:K2 (260 CFU). CONCLUSIONS: Our study has shown that pre-treatment of animals with anti-LPS monoclonal antibodies resulted in 33% survival compared to unprotected animals that all died within 4 days. Beside survival we found also different bacterial loads between two groups.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA