Naslov
Natural sources and chemistry of resveratrol

Autori
Katalinić, Višnja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
5th Croatian Congress of Pharmacology and 2nd Congress of Croatian Phydiological Society with International Participation ; Periodicum Biologorum 109 (2007) (Suppl. 2) 1-182 / Vitale, Branko - Zagreb : Hrvatsko prirodoslovno društvo, 2007, 53-53

Skup
5th Croatian Congress of Pharmacology and 2nd Congress of Croatian Phydiological Society with International Participation

Mjesto i datum
Osijek, Hrvatska, 19.09.2007. - 22.09.2007

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Resveratrol; Natural sources

Sažetak
The link between nutrition and a number of diseases is becoming clearer every day. While the consumer is advised to enrich the nutrition with antioxidative compounds, antioxidant mechanisms are being investigated, natural antioxidant sources identified and bioactive molecules isolated. Resveratrol (3, 5, 4'-trihydroxystilbene) has emerged as one of the key molecules among bioactive phenols, due to its antioxidative, cardioprotective, antimutagenic, antiinflammatory, and especially emphasized, antitumor effect. The number of plants able to synthesize stilbenes is limited. It is mostly present in small amounts, being one of the phytoalexins, synthesized by plants as a response to injury. Although resveratrol has been identified as active ingredient of some appreciated medicinal herbs, such as Polygonum cuspidatum, a plant with special position in traditional medicine of East, its content in our medicinal herbs has not been investigated. It can be supposed that some plants traditionally used in Croatian folk medicine are rich with resveratrol or its closely related conjugates and derivatives which also show biological activity and have potential therapeutic value. The results of screening of 70 medicinal plant extracts confirm that individual medicinal plants can be an important dietary source of phenolic compounds with high antioxidant capacity comparable with red wine or beverages like tea. The presence of free resveratrol monomers was confirmed in individual plant infusions prepared in common way in which teas are prepared for human consumption. During further investigations, extraction of phenolic compounds from plant material was conducted using alcoholic solvents. As the structure-activity relationship examination showed that glycosylation of the stilbenes could implicate with their biological properties, extraction was conducted under mild conditions to protect true structure of phenolic compounds. Polyphenolic composition and antioxidant properties of medicinal plant extracts were analyzed. Free radical scavenging ability and ferric reducing antioxidant power of plant extracts were compared at equal concentration value of total phenols. The separation and quantification of stilbenes by HPLC-RP-PAD, using internal standard method, indicated significant differences in the polyphenolic composition of selected plant extracts. Free resveratrol monomers, if found, were present in very small amounts, mostly as E-isomer. The results of investigations strongly indicate that resveratrol or its hydroxy or methoxy derivatives are present in row plant material as glucosides. Resveratrol derivatives piceid (3, 4′ , 5-trihydroxystilbene-3-β -D-glucoside), astringin (3, 3′ , 4′ , 5-tetrahydroxystilbene-3-β -D-glucoside) and especially isorhapontin (3′ -methoxy-3, 4′ , 5-trihydroxystilbene-3-β -D-glucoside) were confirmed as dominant stilbenes in controlled medicinal plant extracts. Among the controlled plant material the Teucrii montani herba, Salviae folium, and Fragariae folium extracts can be considered the best sources of resveratrol isorhapontin and piceid. As these medicinal plants are well-known in phytomedicine their medicinal properties may be related to presence of these resveratrol derivatives. The results of plant potential research, regarding the content of biologically active stilbenes, could motivate the development of new ways of using resveratrol-rich plant material, and find its application in the food industry (biologically valuable food production), as well as pharmaceutical industry.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA